Xie Cheng-Hui, Yokota Akira
Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo 113-0032, Japan.
Int J Syst Evol Microbiol. 2005 Jul;55(Pt 4):1435-1438. doi: 10.1099/ijs.0.63503-0.
The taxonomic position of the free-living diazotrophic strain COC8(T) isolated from rice was investigated based on phylogenetic analyses. 16S rRNA gene sequence analyses indicated that strain COC8(T) was closely related to the genus Azospirillum (96% similarity). Chemotaxonomic characteristics (G+C content of the DNA 66.8 mol%, Q-10 quinone system, 18:1omega7c as the major fatty acid and 14:0 3-OH and 16:0 3-OH as the major hydroxy fatty acids) were also similar to those of the genus Azospirillum. Based on its physiological characteristics, strain COC8(T) can clearly be differentiated from recognized species of Azospirillum. The name Azospirillum oryzae sp. nov. is proposed to accommodate this bacterial strain; the type strain is COC8(T) (=IAM 15130(T)=CCTCC AB204051(T)).
基于系统发育分析,对从水稻中分离出的自生固氮菌株COC8(T)的分类地位进行了研究。16S rRNA基因序列分析表明,菌株COC8(T)与固氮螺菌属密切相关(相似度为96%)。化学分类特征(DNA的G+C含量为66.8 mol%,醌系统为Q-10,主要脂肪酸为18:1ω7c,主要羟基脂肪酸为14:0 3-OH和16:0 3-OH)也与固氮螺菌属相似。基于其生理特征,菌株COC8(T)可明显与固氮螺菌属的已知种区分开来。提议将该细菌菌株命名为水稻固氮螺菌(Azospirillum oryzae)新种;模式菌株为COC8(T)(=IAM 15130(T)=CCTCC AB204051(T))。