Shivaji S, Gupta Pratima, Chaturvedi Preeti, Suresh K, Delille Daniel
Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India.
Université P. & M. Curie (Paris 6), Observatoire Océanologique de Banyuls, CNRS URA 2071, F-66650 Banyuls sur Mer, France.
Int J Syst Evol Microbiol. 2005 Jul;55(Pt 4):1453-1456. doi: 10.1099/ijs.0.63478-0.
A psychrotolerant, Gram-negative, motile bacterium, designated CK 47(T), was isolated from sea water off the subantarctic Kerguelen islands (50 degrees 40' S 68 degrees 25' E). The isolate grew optimally at 22 degrees C and minimum and maximum temperature of growth were 4 and 37 degrees C, respectively. It required Na(+) for growth and exhibited optimum growth at pH 8.5 and 4% NaCl. It utilized hexane, heptane and petroleum ether as sole sources of carbon. Strain CK 47(T) had Q9 as the major respiratory quinone and C(16 : 0) (21.7%), C(17:0) (21.3%), C(18:0) (5.7%), C(18:1)omega7c (9.0%) and C(18:1)omega9c (31.4%) as predominant fatty acids. The G+C content of the DNA was 58 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that CK 47(T) formed a coherent cluster within the genus Marinobacter. It exhibited highest 16S rRNA gene sequence similarity of 96.8% with Marinobacter lipolyticus. However, the level of DNA-DNA relatedness between strain CK47(T) and M. lipolyticus was only 55%. On the basis of phenotypic characteristics, and phylogenetic and genotypic distinctiveness, strain CK 47(T) is considered to represent a novel species of the genus Marinobacter. The name Marinobacter maritimus sp. nov. is proposed, with CK 47(T) (=JCM 12521(T)=MTCC 6519(T)) as the type strain.
从亚南极凯尔盖朗群岛（南纬50°40′，东经68°25′）附近海域分离出一株耐冷、革兰氏阴性、具运动性的细菌，命名为CK 47(T)。该菌株在22℃生长最佳，最低和最高生长温度分别为4℃和37℃。其生长需要Na(+)，在pH 8.5和4% NaCl条件下生长最佳。它能利用己烷、庚烷和石油醚作为唯一碳源。菌株CK 47(T)的主要呼吸醌为Q9，主要脂肪酸为C(16 : 0)（21.7%）、C(17:0)（21.3%）、C(18:0)（5.7%）、C(18:1)ω7c（9.0%）和C(18:1)ω9c（31.4%）。DNA的G+C含量为58 mol%。基于16S rRNA基因序列的系统发育分析表明，CK 47(T)在海杆菌属内形成一个连贯的聚类。它与解脂海杆菌的16S rRNA基因序列相似性最高，为96.8%。然而，菌株CK47(T)和解脂海杆菌之间的DNA-DNA相关性仅为55%。基于表型特征、系统发育和基因型差异，菌株CK 47(T)被认为代表海杆菌属的一个新物种。提出新物种名称海栖海杆菌，模式菌株为CK 47(T)（=JCM 12521(T)=MTCC 6519(T)）。
