An Dong-Shan, Im Wan-Taek, Yang Hee-Chan, Yang Deok-Chun, Lee Sung-Taik
Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Guseong-dong 373-1, Yuseong-gu, Daejeon 305-701, South Korea.
Department of Oriental Medicinal Material & Processing, College of Life Science, Kyung Hee University, 1 Seocheon, Kihung Yongin, Kyunggi 449-701, South Korea.
Int J Syst Evol Microbiol. 2005 Jul;55(Pt 4):1625-1628. doi: 10.1099/ijs.0.63695-0.
A bacterial strain (designated BB4(T)), which has beta-glucosidase activity, was isolated from soil around the roots of bamboo plants. Cells were Gram-negative, aerobic, non-motile and straight-rod-shaped. Phylogenetic analysis of 16S rRNA gene sequences revealed a clear affiliation with members of the family 'Xanthomonadaceae'. The 16S rRNA gene sequence of strain BB4(T) showed the following sequence similarities: 97.7% to Dyella japonica XD53(T), 97.1% to Frateuria aurantia LMG 1558(T), 96.2% to Fulvimonas soli LMG 19981(T), 94.3% to Rhodanobacter lindaniclasticus RP5575(T) and <90% to other members of the 'Gammaproteobacteria'. The G+C content of the genomic DNA was 63.8 mol%. The major fatty acids were branched forms, especially large proportions of iso-C(15:0), iso-C(17:0) and iso-C(17:1)omega9c, similar to the profile of the genus Dyella. The results of DNA-DNA hybridization with D. japonica XD53(T) and Frateuria aurantia LMG 1558(T), in combination with phenotypic characteristics and 16S rRNA gene sequence analysis, demonstrated that strain BB4(T) should be classified as a novel Dyella species. The name Dyella koreensis sp. nov. is proposed, with strain BB4(T) (=KCTC 12359(T)=NBRC 100831(T)) as the type strain.
从竹类植物根际土壤中分离出一株具有β-葡萄糖苷酶活性的细菌菌株（命名为BB4(T)）。细胞革兰氏阴性，好氧，不运动，呈直杆状。16S rRNA基因序列的系统发育分析表明，它与“黄单胞菌科”的成员有明显的亲缘关系。菌株BB4(T)的16S rRNA基因序列显示出以下序列相似性：与日本迪茨氏菌XD53(T)相似性为97.7%，与橙色弗氏菌LMG 1558(T)相似性为97.1%，与土壤黄单胞菌LMG 19981(T)相似性为96.2%，与林丹分解红杆菌RP5575(T)相似性为94.3%，与“γ-变形菌纲”的其他成员相似性小于90%。基因组DNA的G+C含量为63.8 mol%。主要脂肪酸为支链形式，尤其是异-C(15:0)、异-C(17:0)和异-C(17:1)ω9c比例较高，类似于迪茨氏菌属的特征。与日本迪茨氏菌XD53(T)和橙色弗氏菌LMG 1558(T)的DNA-DNA杂交结果，结合表型特征和16S rRNA基因序列分析，表明菌株BB4(T)应被归类为一个新的迪茨氏菌物种。提出新物种名称韩国迪茨氏菌（Dyella koreensis sp. nov.），以菌株BB4(T)（=KCTC 12359(T)=NBRC 100831(T)）作为模式菌株。
