De Clercq Deborah, Van Trappen Stefanie, Cleenwerck Ilse, Ceustermans An, Swings Jean, Coosemans Jozef, Ryckeboer Jaak
Laboratory of Phytopathology and Plant Protection, Katholieke Universiteit Leuven, W. De Croylaan 42, B-3001 Leuven, Belgium.
BCCM/LMG Bacteria Collection, Laboratory of Microbiology, University of Ghent, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium.
Int J Syst Evol Microbiol. 2006 Aug;56(Pt 8):1755-1759. doi: 10.1099/ijs.0.64131-0.
Three Gram-negative, yellow-pigmented strains were isolated from the rhizospheres of Spathiphyllum plants grown in a compost-amended potting mix. The strains showed biological control activity towards the root-rot plant pathogen Cylindrocladium spathiphylli, and were characterized to determine their taxonomic position. Cells of the strains were non-motile rods, and the strains were oxidase- and catalase-positive and unable to ferment most sugars tested. The three strains showed differences in growth temperature range, optimal growth temperature and some biochemical reactions. The majority of the fatty acids were branched, and large amounts of 15 : 0 iso and 17 : 1 iso omega9c were present. The 16S rRNA gene sequence (1,497 bp) of strain B39(T) showed the highest level of similarity (98.5 %) to that of Rhodanobacter fulvus IAM 15025(T), followed by Rhodanobacter lindaniclasticus LMG 18385(T) (96.0 %; strain no longer extant), Dyella koreensis CCUG 50883(T) (96.4 %), Dyella japonica DSM 16301(T) (96.3 %), Frateuria aurantia LMG 1558(T) (96.2 %) and Fulvimonas soli LMG 19981(T) (95.9 %). Less than 90 % 16S rRNA gene sequence similarity was observed for other members of the Gammaproteobacteria. The mean DNA-DNA reassociation value for the three strains was 100 % and was 25 % when the strains were compared with DNA from R. fulvus LMG 23003(T). The strains had a mean DNA G+C content of 67.6 mol%. On the basis of their phylogenetic, genomic and phenotypic properties, the three strains represent a novel species within the genus Rhodanobacter, for which the name Rhodanobacter spathiphylli sp. nov. is proposed. The type strain is strain B39(T) (=LMG 23181(T)=DSM 17631(T)).
从种植于添加了堆肥的盆栽基质中的白鹤芋植物根际分离出三株革兰氏阴性、产黄色色素的菌株。这些菌株对根腐病植物病原菌马蹄莲柱枝霉表现出生物防治活性，并对其进行了特征分析以确定它们的分类地位。菌株细胞为无动力杆菌，菌株氧化酶和过氧化氢酶呈阳性，且不能发酵大多数测试糖类。这三株菌株在生长温度范围、最适生长温度和一些生化反应方面存在差异。大多数脂肪酸为支链，且存在大量的15 : 0异戊酸和17 : 1异戊酸ω9c。菌株B39(T)的16S rRNA基因序列（1497 bp）与黄红杆菌IAM 15025(T)的序列相似度最高（98.5%），其次是林丹降解黄红杆菌LMG 18385(T)（96.0%；该菌株已不存在）、韩国迪茨氏菌CCUG 50883(T)（96.4%）、日本迪茨氏菌DSM 16301(T)（96.3%）、aurantia弗氏菌LMG 1558(T)（96.2%）和土壤富黄单胞菌LMG 19981(T)（95.9%）。对于γ-变形菌纲的其他成员，观察到的16S rRNA基因序列相似度低于90%。这三株菌株的平均DNA-DNA重结合值为100%，与黄红杆菌LMG 23003(T)的DNA相比时为25%。这些菌株的平均DNA G+C含量为67.6 mol%。基于它们的系统发育、基因组和表型特征，这三株菌株代表黄红杆菌属内的一个新物种，为此提出黄红杆菌白鹤芋新种（Rhodanobacter spathiphylli sp. nov.）。模式菌株为菌株B39(T)（=LMG 23181(T)=DSM 17631(T)）。
