Saha P, Krishnamurthi S, Mayilraj S, Prasad G S, Bora T C, Chakrabarti T
Microbial Type Culture Collection & Gene Bank (MTCC), Institute of Microbial Technology, Sector 39A, Chandigarh - 160 036, India.
Biotechnology Division, Regional Research Laboratory, Jorhat - 785 006, Assam, India.
Int J Syst Evol Microbiol. 2005 Jul;55(Pt 4):1491-1495. doi: 10.1099/ijs.0.63552-0.
A bacterial strain designated GPTSA 20(T), which was isolated from a warm spring in Assam, India, was characterized by using a polyphasic approach. The cells were Gram-negative, aerobic rods, which could not utilize or produce acid from most of the carbohydrates tested. The predominant fatty acids were C(15:0) iso (25.04%), C(17:1) iso omega9c (19.28%), C(16:0) iso (17.73%) and C(11:0) iso 3-OH (9.34%). The G+C content was 75 mol%. From 16S rRNA gene sequence analysis (1433 nucleotides, continuous stretch), it was confirmed that strain GPTSA 20(T) belonged to the class 'Gammaproteobacteria'. The closest 16S rRNA gene sequence similarity found (98.2%) was with an uncultured bacterium clone, NB-03 (accession no. AB117707), from an autotrophic nitrifying biofilm. Among culturable bacteria, the closest sequence similarities were with Fulvimonas soli (93.0%), Silanimonas lenta (92.8%), Thermomonas hydrothermalis (92.4%), Frateuria aurantia (91.9%), Rhodanobacter lindaniclasticus (91.9%), Thermomonas haemolytica (91.9%) and Pseudoxanthomonas taiwanensis (91.8%); similarities of less than 91.8% were obtained with other members of the class 'Gammaproteobacteria'. From the biochemical, physiological, chemotaxonomic and phylogenetic analysis, it was clear that strain GPTSA 20(T) was quite different from members of known genera of the class 'Gammaproteobacteria'. Therefore, it is proposed that strain GPTSA 20(T) represents a novel species within a new genus, with the name Aquimonas voraii gen. nov., sp. nov. The type strain is GPTSA 20(T) (=MTCC 6713(T)=JCM 12896(T)).
从印度阿萨姆邦的一处温泉中分离出的一株细菌，命名为GPTSA 20(T)，采用多相分类法对其进行了特征描述。该菌细胞为革兰氏阴性、需氧杆菌，不能利用所测试的大多数碳水化合物产酸或从中产酸。主要脂肪酸为异C(15:0)（25.04%）、异C(17:1) ω9c（19.28%）、异C(16:0)（17.73%）和异C(11:0) 3-OH（9.34%）。G+C含量为75 mol%。通过16S rRNA基因序列分析（1433个核苷酸，连续片段），证实菌株GPTSA 20(T)属于“γ-变形菌纲”。发现的最相近的16S rRNA基因序列相似性（98.2%）是与来自自养硝化生物膜的一株未培养细菌克隆NB-03（登录号AB117707）。在可培养细菌中，最相近的序列相似性分别与土壤富黄单胞菌（93.0%）、迟缓硅单胞菌（92.8%）、嗜热栖热单胞菌（92.4%）、aurantia弗拉第菌（91.9%）、林丹降解红杆菌（91.9%）、溶血栖热单胞菌（91.9%）和台湾假黄单胞菌（91.8%）；与“γ-变形菌纲”的其他成员的相似性均低于91.8%。从生化、生理、化学分类和系统发育分析来看，很明显菌株GPTSA 20(T)与“γ-变形菌纲”已知属的成员有很大不同。因此，建议菌株GPTSA 20(T)代表一个新属内的一个新物种，命名为沃氏水生菌属（Aquimonas），新种（voraii）。模式菌株为GPTSA 20(T)（=MTCC 6713(T)=JCM 12896(T)）。
