van 't Wout Angélique B, Swain J Victor, Schindler Michael, Rao Ushnal, Pathmajeyan Melissa S, Mullins James I, Kirchhoff Frank
Department of Microbiology, University of Washington School of Medicine, Seattle, 98195, USA.
J Virol. 2005 Aug;79(15):10053-8. doi: 10.1128/JVI.79.15.10053-10058.2005.
Several recent reports indicate that cholesterol might play an important role in human immunodeficiency virus type 1 (HIV-1) replication. We investigated the effects of HIV-1 infection on cholesterol biosynthesis and uptake using microarrays. HIV-1 increased gene expression of cholesterol genes in both transformed T-cell lines and primary CD4(+) T cells. Consistent with our microarray data, (14)C-labeled mevalonate and acetate incorporation was increased in HIV-1-infected cells. Our data also demonstrate that changes in cholesterol biosynthesis and uptake are only observed in the presence of functional Nef, suggesting that increased cholesterol synthesis may contribute to Nef-mediated enhancement of virion infectivity and viral replication.
最近的几份报告表明，胆固醇可能在1型人类免疫缺陷病毒（HIV-1）复制中发挥重要作用。我们使用微阵列研究了HIV-1感染对胆固醇生物合成和摄取的影响。HIV-1增加了转化T细胞系和原代CD4(+) T细胞中胆固醇基因的表达。与我们的微阵列数据一致，HIV-1感染细胞中(14)C标记的甲羟戊酸和乙酸掺入增加。我们的数据还表明，仅在功能性Nef存在的情况下才观察到胆固醇生物合成和摄取的变化，这表明胆固醇合成增加可能有助于Nef介导的病毒体感染性增强和病毒复制。
