Walker J D, Martin M A, Potts D G, Lemaire C, Templeton D M
Magnetic Resonance Laboratory, Toronto Western Hospital, Canada.
Invest Radiol. 1992 Apr;27(4):298-301. doi: 10.1097/00004424-199204000-00008.
The authors used magnetic resonance imaging (MRI) techniques to examine the effect of meningeal trauma produced by cisterna magna puncture on the integrity of the blood-cerebrospinal fluid barrier (BCB) in a rat model.
Intravenous gadolinium-DTPA (Gd-DTPA), a relaxation rate modifier which normally does not cross the BCB, was used as a probe to follow leakage of fluid across the BCB. After Gd-DTPA injection, cerebrospinal fluid (CSF) serial samples were obtained through the needle used to create the experimental trauma. These samples were subsequently examined in vitro by MRI to obtain their T1 relaxation rates and assayed by mass spectrometry for gadolinium and elemental iron concentrations.
The iron levels reflected the severity of puncture-related subarachnoid hemorrhage. Rats with ongoing meningeal damage showed significantly higher CSF levels of gadolinium and significantly higher CSF T1 relaxation rates than controls at all samples times over 1 hour after the puncture. Blood in the CSF could not explain these changes because the CSF iron levels did not significantly differ from control levels.
Intravenously administered Gd-DTPA can gain access to the subarachnoid space through minor defects in the BCB and cause significant increases in CSF T1 relaxation rates.
作者利用磁共振成像（MRI）技术，在大鼠模型中研究了枕大池穿刺造成的脑膜创伤对血脑脊液屏障（BCB）完整性的影响。
静脉注射钆喷酸葡胺（Gd-DTPA），一种通常不会穿过血脑脊液屏障的弛豫率调节剂，用作追踪液体穿过血脑脊液屏障渗漏情况的探针。注射Gd-DTPA后，通过用于造成实验性创伤的针获取脑脊液（CSF）系列样本。随后对这些样本进行体外MRI检查以获得其T1弛豫率，并通过质谱法测定钆和元素铁的浓度。
铁水平反映了穿刺相关蛛网膜下腔出血的严重程度。在穿刺后1小时以上的所有采样时间点，持续存在脑膜损伤的大鼠脑脊液中的钆水平显著更高，脑脊液T1弛豫率也显著高于对照组。脑脊液中的血液无法解释这些变化，因为脑脊液铁水平与对照水平无显著差异。
静脉注射的Gd-DTPA可通过血脑脊液屏障的微小缺陷进入蛛网膜下腔，并导致脑脊液T1弛豫率显著升高。
