Shao Yuan-Jian, Chen Zong-Xiang, Zhang Ya-Fang, Chen En-Hui, Qi Ding-Cheng, Miao Jin, Pan Xue-Biao
Agricultural College of Yangzhou University, Yangzhou 225009, China.
Yi Chuan Xue Bao. 2005 May;32(5):501-6.
A clonally propagated F2 population (F2CP), derived from the rice cross of Qimiaoxiang/91SP068, was used to map rolled leaf QTLs. As the parent Qimiaoxiang is an unrolling leaf variety, while 91SP068 is a medium rolling variety with about 34% rolling index. One major QTL, rl8, which came from 91SP068, was mapped between two flanking SSR markers, RM6954 and RM6841, on chromosome 5, with genetic distance 3.8 cM, and was 1.0 cM away from RM6954. Its additive effect estimated by composite interval mapping (CIM) was 9.61 in 2002 and 6.23 in 2003, and the dominance effect was also different between two years, -1.19 in 2002 and -4.44 in 2003, respectively. It explained about 20% - 33% of the total phenotypic variation between two years. Furthermore, an integrated physical and genetic map encompassing rl8 region was constructed, and the physical distance of the interval was 542 kb, and the ratio of physical to genetic distance was 144 kb/cM. Based on this research, fine mapping of rolled leaf QTLs will not only facilitate the map-based cloning of the gene itself, but also improve the efficiency of marker-assisted selection in plant breeding.
利用从水稻品种七妙香与91SP068杂交后代中衍生出的一个克隆繁殖的F2群体（F2CP）来定位卷叶QTL。由于亲本七妙香是一个叶片不卷曲的品种，而91SP068是一个中度卷叶品种，卷叶指数约为34%。一个来自91SP068的主效QTL，rl8，被定位在第5染色体上的两个侧翼SSR标记RM6954和RM6841之间，遗传距离为3.8 cM，且与RM6954相距1.0 cM。通过复合区间作图（CIM）估计其在2002年的加性效应为9.61，在2003年为6.23，两年间的显性效应也不同，分别为2002年的-1.19和2003年的-4.44。它解释了两年间总表型变异的约20% - 33%。此外，构建了一个包含rl8区域的物理和遗传整合图谱，该区间的物理距离为542 kb，物理与遗传距离的比率为144 kb/cM。基于这项研究，卷叶QTLs的精细定位不仅将有助于该基因本身的图位克隆，还将提高植物育种中标记辅助选择的效率。
