Gorbet Maud B, Sefton Michael V
Department of Chemical Engineering, McMaster University, Hamilton, Ontario, Canada.
Biomaterials. 2005 Dec;26(34):6811-7. doi: 10.1016/j.biomaterials.2005.04.063.
In the laboratory environment where biomaterials are synthesized and their biocompatibility assessed, we find that endotoxin contamination is hard to avoid and must not be ignored. In those relatively few cases where endotoxin was known to be present, it has been clearly shown that endotoxin can significantly affect the biological response observed and hence confound any effect of the material. This short review explains what endotoxin is, how to test for it and remove it and what its effect on the biological response to biomaterials is. We advocate routine testing of endotoxin on biomaterials and of reagents used in experimental evaluation of biomaterials and this should be the responsibility of every scientist to ensure the validity of any biomaterial study.
在合成生物材料并评估其生物相容性的实验室环境中，我们发现内毒素污染难以避免且不容忽视。在那些已知存在内毒素的相对少数情况下，已清楚表明内毒素会显著影响所观察到的生物学反应，从而混淆材料的任何作用。这篇简短综述解释了什么是内毒素、如何检测和去除内毒素以及它对生物材料生物学反应的影响。我们主张对生物材料以及用于生物材料实验评估的试剂进行内毒素常规检测，每位科学家都应负责确保任何生物材料研究的有效性。
