Visanji James M, Thompson David G, Padfield Philip J
Section of Gastrointestinal Science, Department of Medicine, University of Manchester, Clinical Sciences Building, Hope Hospital, Stott Lane, Salford M6 8HD, UK.
Cancer Lett. 2006 Jun 8;237(1):130-6. doi: 10.1016/j.canlet.2005.05.045. Epub 2005 Jul 14.
Carnosol and carnosic acid, two antioxidant polyphenols present in Rosmarinus officinalis (rosemary), were investigated for their antiproliferative properties toward Caco-2 cells. Twenty hours of treatment with both carnosol and carnosic acid inhibited 3H-thymidine incorporation in a dose-dependent manner, with a 50% inhibitory concentration of 23 microM and significantly increased the doubling time of Caco-2 cells from 29.5 to 140 and 120 h, respectively. These effects were associated with accumulation of treated cells in the G2/M phase of the cell cycle. Carnosol was found to exert its major cell cycle effect after prometaphase, and caused an increase in cyclin B1 protein levels whereas carnosic acid arrested cells prior to prometaphase, and caused a reduction in cyclin A levels. These structurally related phytochemicals, therefore, appear to arrest cells at different phases of the cell cycle possibly through influencing the levels of different cyclin proteins.
研究了迷迭香（Rosmarinus officinalis）中存在的两种抗氧化多酚——鼠尾草酸和鼠尾草酚对Caco-2细胞的抗增殖特性。用鼠尾草酸和鼠尾草酚处理20小时均以剂量依赖方式抑制3H-胸腺嘧啶核苷掺入，50%抑制浓度为23微摩尔，并且使Caco-2细胞的倍增时间分别从29.5小时显著增加到140小时和120小时。这些效应与处理后的细胞在细胞周期的G2/M期积累有关。发现鼠尾草酸在前中期之后发挥其主要的细胞周期效应，并导致细胞周期蛋白B1水平升高，而鼠尾草酚在前中期之前使细胞停滞，并导致细胞周期蛋白A水平降低。因此，这些结构相关的植物化学物质似乎可能通过影响不同细胞周期蛋白的水平使细胞停滞在细胞周期的不同阶段。
