Iredale J, Wainer I W
Department of Oncology, McGill University, Montreal, Quebec, Canada.
J Chromatogr. 1992 Jan 17;573(2):253-8. doi: 10.1016/0378-4347(92)80126-b.
A sequential achiral-chiral high-performance liquid chromatographic system has been developed for the determination of the enantiomers of hydroxychloroquine, (+)-HCQ and (-)-HCQ, and the enantiomers of its three major metabolites, bisdesethylchloroquine, desethylhydroxychloroquine and desethylchloroquine, in plasma. The HCQ was separated from the metabolites and interfering components in the plasma and quantified on a cyano-bonded phase, and the enantiomeric composition determined using a Chiral-AGP chiral stationary phase. The assay was validated and applied to the analysis of a pilot study of the pharmacokinetics of (+)- and (-)-HCQ in rabbits.
已开发出一种顺序非手性-手性高效液相色谱系统，用于测定血浆中羟氯喹（HCQ）对映体（+）-HCQ和（-）-HCQ及其三种主要代谢物双去乙基氯喹、去乙基羟氯喹和去乙基氯喹的对映体。HCQ与血浆中的代谢物及干扰成分分离，并在氰基键合相上进行定量，使用Chiral-AGP手性固定相测定对映体组成。该分析方法经过验证，并应用于兔体内（+）-和（-）-HCQ药代动力学的初步研究分析。
