利用随机扩增多态性DNA（RAPD）和28S核糖体DNA测序分析巴西副球孢子菌和脑形副球孢子菌“摩尔”的遗传多态性——对脑形副球孢子菌的重新审视

Analysis of the genetic polymorphism of Paracoccidioides brasiliensis and Paracoccidioides cerebriformis "Moore" by random amplified polymorphic DNA (RAPD) and 28S ribosomal DNA sequencing--Paracoccidioides cerebriformis revisited.

作者信息

Cavalcanti Sarah Desirée Barbosa, Levi José Eduardo, Dantas Kátia Cristina, Martins José Eduardo Costa

机构信息

Laboratório de Micologia Médica, Instituto de Medicina Tropical de São Paulo, SP, Brasil.

出版信息

Rev Inst Med Trop Sao Paulo. 2005 May-Jun;47(3):119-23. doi: 10.1590/s0036-46652005000300001. Epub 2005 Jul 12.

DOI:10.1590/s0036-46652005000300001

PMID:16021283

Abstract

Our purpose was to compare the genetic polymorphism of six samples of P. brasiliensis (113, 339, BAT, T1F1, T3B6, T5LN1), with four samples of P. cerebriformis (735, 741, 750, 361) from the Mycological Laboratory of the Instituto de Medicina Tropical de São Paulo, using Random Amplified Polymorphic DNA Analysis (RAPD). RAPD profiles clearly segregated P. brasiliensis and P. cerebriformis isolates. However, the variation on band patterns among P. cerebriformis isolates was high. Sequencing of the 28S rDNA gene showed nucleotide conservancy among P. cerebriformis isolates, providing basis for taxonomical grouping, and disclosing high divergence to P. brasiliensis supporting that they are in fact two distinct species. Moreover, DNA sequence suggests that P. cerebriformis belongs in fact to the Aspergillus genus.

摘要

我们的目的是使用随机扩增多态性DNA分析（RAPD），比较来自圣保罗热带医学研究所真菌学实验室的6个巴西副球孢子菌样本（113、339、BAT、T1F1、T3B6、T5LN1）与4个脑状副球孢子菌样本（735、741、750、361）的遗传多态性。RAPD图谱清晰地将巴西副球孢子菌和脑状副球孢子菌分离株区分开来。然而，脑状副球孢子菌分离株之间的条带模式变化很大。28S rDNA基因测序显示脑状副球孢子菌分离株之间存在核苷酸保守性，为分类分组提供了依据，并揭示了与巴西副球孢子菌的高度差异，支持它们实际上是两个不同的物种。此外，DNA序列表明脑状副球孢子菌实际上属于曲霉属。