通过核糖体DNA的PCR扩增对巴西副球孢子菌进行分子鉴定。
Molecular identification of Paracoccidioides brasiliensis by PCR amplification of ribosomal DNA.
作者信息
Motoyama A B, Venancio E J, Brandão G O, Petrofeza-Silva S, Pereira I S, Soares C M, Felipe M S
机构信息
Laboratório de Biologia Molecular, Instituto de Biologia, Universidade de Brasília, 70910-900, Brasília, DF, Brazil.
出版信息
J Clin Microbiol. 2000 Aug;38(8):3106-9. doi: 10.1128/JCM.38.8.3106-3109.2000.
Abstract
We have amplified and sequenced the 5.8S and 28S ribosomal DNA genes and intergenic regions of Paracoccidioides brasiliensis, strain Pb01. Using primers specifically designed for both ribosomal DNA regions, we were able to discriminate between P. brasiliensis and other human pathogenic fungi by PCR. The use of this molecular marker could be important for paracoccidiodomycosis diagnosis and ecological and molecular epidemiological studies of P. brasiliensis in Latin America.
摘要
我们对巴西副球孢子菌Pb01菌株的5.8S和28S核糖体DNA基因及基因间区域进行了扩增和测序。使用专门针对两个核糖体DNA区域设计的引物，我们能够通过PCR区分巴西副球孢子菌和其他人类致病真菌。这种分子标记的应用对于拉丁美洲副球孢子菌病的诊断以及巴西副球孢子菌的生态和分子流行病学研究可能具有重要意义。
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