Xu Changxin, Wang Sanying, Ren Haixia, Lin Xiangmin, Wu Lina, Peng Xuanxian
Center for Proteomics, Department of Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian 361005, P.R. China.
Proteomics. 2005 Aug;5(12):3142-52. doi: 10.1002/pmic.200401128.
The ability of osmoregulation is crucial to marine pathogens that always face the change of osmotic pressure when they shift between natural marine water-bodies and hosts. Previous studies indicated that the expressional patterns of outer membrane proteins (OMPs) changed when Gram-negative bacteria were transferred in different environments. In the present study, proteomic methodologies were used to investigate the expressional pattern of OMPs of Vibrio alginolyticus, a universal marine pathogen, at different Na(+) concentrations. OmpW, OmpV, and Omp TolC were determined to be osmotic stress responsive proteins. Of the three proteins, importantly, OmpV and OmpW showed distinctly reverse changes to each other, indicating that the two proteins might be the two components varied with changed NaCl concentrations. In addition, our results suggest that closely related species of bacteria with available whole genomic databases should be applied after item microorganism species was used when proteins from a bacterium with unavailable whole genomic information were identified by PMF. Therefore, our results not only expand our knowledge on osmotic stress responsive proteins, but also provide valuable information for strategies on screening of these proteins.
渗透调节能力对于海洋病原体至关重要，因为当它们在天然海水水体和宿主之间转换时，总是面临渗透压的变化。先前的研究表明，当革兰氏阴性菌在不同环境中转移时，外膜蛋白（OMPs）的表达模式会发生变化。在本研究中，蛋白质组学方法被用于研究一种常见的海洋病原体——溶藻弧菌在不同Na⁺浓度下OMPs的表达模式。确定OmpW、OmpV和Omp TolC为渗透应激反应蛋白。重要的是，在这三种蛋白质中，OmpV和OmpW表现出明显相反的变化，这表明这两种蛋白质可能是随NaCl浓度变化而变化的两个组分。此外，我们的结果表明，当通过肽质量指纹图谱（PMF）鉴定来自没有完整基因组信息的细菌的蛋白质时，在使用目标微生物物种之后，应应用具有可用完整基因组数据库的密切相关细菌物种。因此，我们的结果不仅扩展了我们对渗透应激反应蛋白的认识，还为筛选这些蛋白质的策略提供了有价值的信息。
