Pandey Rajkishor, Sharma Simran, Sinha Kislay Kumar
Department of Biotechnology, National Institute of Pharmaceutical Education and Research, Hajipur 844102, Bihar, India.
School of Medicine, University of Missouri, Columbia, MO 65211, USA.
Antibiotics (Basel). 2023 Jun 16;12(6):1062. doi: 10.3390/antibiotics12061062.
The outbreak of waterborne diseases such as cholera and non-cholera (vibriosis) is continuously increasing in the environment due to fecal and sewage discharge in water sources. Cholera and vibriosis are caused by different species of genus which are responsible for acute diarrheal disease and soft tissue damage. Although incidences of cholera and vibriosis have been reported from the Vaishali district of Bihar, India, clinical or environmental strains have not been characterized in this region. Out of fifty environmental water samples, twelve different biochemical test results confirmed the presence of twenty isolates. The isolates were found to belong to five different species, namely , , , , and . From the identified isolates, 65% and 45% isolates were found to be resistant to ampicillin and cephalexin, respectively. Additionally, two isolates were found to be resistant against six and four separately selected antibiotics. Furthermore, virulent and genes were detected by PCR in two different isolates. Additionally, phage induction was also noticed in two different isolates which carry lysogenic phage in their genome. Overall, the results reported the identification of five different species in environmental water samples. The isolates showed multiple antibacterial resistance, phage induction, and virulence gene profile in their genome.
由于水源中粪便和污水的排放，霍乱和非霍乱（弧菌病）等水源性疾病在环境中的爆发持续增加。霍乱和弧菌病由不同种类的弧菌属引起，这些弧菌会导致急性腹泻病和软组织损伤。尽管印度比哈尔邦的瓦伊沙利地区已报告了霍乱和弧菌病的发病率，但该地区尚未对临床或环境菌株进行特征描述。在五十个环境水样中，十二种不同的生化测试结果证实了二十株分离株的存在。这些分离株被发现属于五个不同的物种，即 、 、 、 和 。在所鉴定的分离株中，分别有65%和45%的分离株对氨苄青霉素和头孢氨苄耐药。此外，发现两株分离株分别对六种和四种单独选择的抗生素耐药。此外，通过PCR在两株不同的分离株中检测到了毒力 和 基因。此外，在两株基因组中携带溶原性噬菌体的不同分离株中也观察到了噬菌体诱导现象。总体而言，结果报告了在环境水样中鉴定出五个不同的 物种。这些分离株在其基因组中表现出多重抗菌耐药性、噬菌体诱导和毒力基因特征。
