Asano Keiji G, Ford Michael J, Tomkins Bruce A, Van Berkel Gary J
Organic and Biological Mass Spectrometry Group, Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6131, USA.
Rapid Commun Mass Spectrom. 2005;19(16):2305-12. doi: 10.1002/rcm.2062.
A self-aspirating heated nebulizer probe is described and demonstrated for use in the direct analysis of analytes on surfaces and in liquid samples by atmospheric pressure chemical ionization (APCI) mass spectrometry. Functionality and performance of the probe as a self-aspirating APCI source is demonstrated using reserpine and progesterone as test compounds. The utility of the probe to sample analytes directly from surfaces was demonstrated first by scanning development lanes of a reversed-phase thin-layer chromatography plate in which a three-component dye mixture, viz., Fat Red 7B, Solvent Green 3, and Solvent Blue 35, was spotted and the components were separated. Development lanes were scanned by the sampling probe operated under computer control (x, y plane) while full-scan mass spectra were recorded using a quadrupole ion trap mass spectrometer. In addition, the ability to sample the surface of pharmaceutical tablets (viz., Extra Strength Tylenol and Evista tablets) and to detect the active ingredients (acetaminophen and raloxifene, respectively) selectively was demonstrated using tandem mass spectrometry (MS/MS). Finally, the capability to sample analyte solutions from the wells of a 384-well microtiter plate and to perform quantitative analyses using MS/MS detection was illustrated with cotinine standards spiked with cotinine-d3 as an internal standard.
描述并展示了一种自吸式加热雾化器探头，用于通过大气压化学电离（APCI）质谱直接分析表面和液体样品中的分析物。以利血平和孕酮作为测试化合物，展示了该探头作为自吸式APCI源的功能和性能。首先通过扫描反相薄层色谱板的展开通道来证明该探头直接从表面采样分析物的效用，在该色谱板上点样了一种三组分染料混合物，即油红7B、溶剂绿3和溶剂蓝35，并对各组分进行了分离。在计算机控制下（x、y平面）操作采样探头扫描展开通道，同时使用四极杆离子阱质谱仪记录全扫描质谱。此外，使用串联质谱（MS/MS）证明了对药片片剂（即强效泰诺片和易维特片）表面进行采样并选择性检测活性成分（分别为对乙酰氨基酚和雷洛昔芬）的能力。最后，以添加了可替宁 - d3作为内标的可替宁标准品为例，说明了从384孔微量滴定板的孔中采样分析物溶液并使用MS/MS检测进行定量分析的能力。
