Higashi K, Naito M, Takeya M, Ando M, Araki S, Takahashi K
First Department of Internal Medicine, Kumamoto University Medical School, Japan.
J Leukoc Biol. 1992 May;51(5):444-54. doi: 10.1002/jlb.51.5.444.
Development, differentiation, and distribution of macrophages in fetal rat lungs were investigated immunohistochemically using anti-rat macrophage monoclonal antibodies. In the lung buds, RM-1+ macrophages were first detected on fetal day 13, and some showed reactivity for TRPM-2. They populated in the peribronchial mesenchyme of the lung buds, proliferated in loco, and showed no peroxidase activity in any intracellular organelles. Their immunophenotypic and ultrastructural features were consistent with those of primitive/fetal macrophages. By fetal day 16, some of them expressed ED1, but ED1+ cells were a minor subpopulation throughout the fetal period. On fetal day 18, ED2+ macrophages developed; some also were positive for RM-1, but the others were negative. Both the RM-1+ and ED2+ macrophages were major macrophage subpopulations and expressed Ki-M2R and/or TRPM-3; ED2+ and/or Ki-M2R+ cells are regarded as pulmonary interstitial resident macrophages. In organ culture, a similar expression of differentiation antigens by macrophages was confirmed. None of these macrophages cytochemically showed any peroxidase activity in vivo or in vitro. In the fetal stage, both RM-1+ and ED2+ macrophage subpopulations showed proliferative potential, suggesting their ability to proliferate and survive in vivo.
使用抗大鼠巨噬细胞单克隆抗体，通过免疫组织化学方法研究了胎鼠肺中巨噬细胞的发育、分化和分布。在肺芽中，RM-1+巨噬细胞于胚胎第13天首次被检测到，部分细胞对TRPM-2有反应。它们聚集在肺芽的支气管周围间充质中，在局部增殖，且在任何细胞内细胞器中均无过氧化物酶活性。其免疫表型和超微结构特征与原始/胎儿巨噬细胞一致。到胚胎第16天，部分细胞表达ED1，但在整个胎儿期ED1+细胞都是少数亚群。胚胎第18天，ED2+巨噬细胞出现；部分细胞对RM-1也呈阳性，但其他细胞为阴性。RM-1+和ED2+巨噬细胞均为主要的巨噬细胞亚群，并表达Ki-M2R和/或TRPM-3；ED2+和/或Ki-M2R+细胞被视为肺间质驻留巨噬细胞。在器官培养中，证实巨噬细胞有类似的分化抗原表达。这些巨噬细胞在体内或体外的细胞化学检测中均未显示任何过氧化物酶活性。在胎儿阶段，RM-1+和ED2+巨噬细胞亚群均显示出增殖潜能，表明它们在体内具有增殖和存活的能力。
