Bagnyukova Tetyana V, Vasylkiv Olena Yu, Storey Kenneth B, Lushchak Volodymyr I
Department of Biochemistry, Institute of Natural Sciences, Vassyl Stefanyk Precarpathian National University, Ivano-Frankivsk, Ukraine.
Brain Res. 2005 Aug 9;1052(2):180-6. doi: 10.1016/j.brainres.2005.06.002.
The effects of in vivo inhibition of catalase by 3-amino 1,2,4-triazole (AMT) on the levels of damage products resulting from reactive oxygen species attack on proteins and lipids as well as on the activities of five antioxidant and associated enzymes were studied in the brain of goldfish, Carassius auratus. Intraperitoneal injection of AMT at a concentration of 0.1 mg/g wet weight caused a gradual decrease in brain catalase activity over 72 h, whereas higher AMT concentrations (0.5 or 1.0 mg/g) reduced catalase activity by about two-thirds within 5-10 h. AMT effects on antioxidant enzyme activities and oxidative stress markers were studied in detail using fish treated with 0.5 mg/g AMT for 24 or 168 h. The levels of thiobarbituric acid-reactive substances (a lipid damage product) increased 6.5-fold by 24 h after AMT injection but fell again after 168 h. The content of carbonylproteins (CP) also rose within 24 h (by approximately 2-fold) and remained 1.5-fold higher compared with respective sham-injected fish after 168 h. CP levels correlated inversely with catalase activity (R(2) = 0.83) suggesting that catalase may protect proteins in vivo against oxidative modification. The activities of both glutathione peroxidase and glutathione-S-transferase increased by approximately 50% and 80%, respectively, in brain of AMT-treated fish and this might represent a compensatory response to lowered catalase activity. Possible functions of catalase in the maintenance of prooxidant/antioxidant balance in goldfish brain are discussed.
研究了在金鱼（Carassius auratus）脑中，3-氨基-1,2,4-三唑（AMT）对过氧化氢酶的体内抑制作用，以及活性氧攻击蛋白质和脂质所产生的损伤产物水平，同时研究了五种抗氧化及相关酶的活性。腹腔注射浓度为0.1 mg/g湿重的AMT后，金鱼脑过氧化氢酶活性在72小时内逐渐降低，而较高浓度的AMT（0.5或1.0 mg/g）在5 - 10小时内使过氧化氢酶活性降低约三分之二。使用经0.5 mg/g AMT处理24或168小时的金鱼，详细研究了AMT对抗氧化酶活性和氧化应激标志物的影响。注射AMT后24小时，硫代巴比妥酸反应性物质（一种脂质损伤产物）的水平增加了6.5倍，但在168小时后又下降。羰基蛋白（CP）含量在24小时内也有所上升（约2倍），168小时后与相应假注射金鱼相比仍高1.5倍。CP水平与过氧化氢酶活性呈负相关（R(2) = 0.83），这表明过氧化氢酶可能在体内保护蛋白质免受氧化修饰。在经AMT处理的金鱼脑中，谷胱甘肽过氧化物酶和谷胱甘肽-S-转移酶的活性分别增加了约50%和80%，这可能是对过氧化氢酶活性降低的一种代偿反应。本文讨论了过氧化氢酶在维持金鱼脑内促氧化剂/抗氧化剂平衡中的可能作用。
