Mo Weiwei, Wu Xianjian, Jia Gang, Zhao Hua, Chen Xiaoling, Tang Jiayong, Wu Caimei, Cai Jingyi, Tian Gang, Wang Jing, Liu Gangmang
Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, China.
Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Chengdu 611130, China.
Anim Nutr. 2018 Sep;4(3):322-328. doi: 10.1016/j.aninu.2018.02.003. Epub 2018 Mar 3.
This study evaluated the effects of arginine (Arg) or N-carbamylglutamate (NCG) on inflammation, antioxidant property, and antioxidant-related gene expression in rat spleen under oxidative stress. A total of 52 rats were randomly distributed into 4 treatment groups with 13 replicates per group. Rats were fed a basal diet (BD) or BD supplemented with Arg or NCG for 30 days. On day 28, half of the BD-fed rats were intraperitoneally injected with sterile saline (control group), and the other half with 12 mg/kg body weight of diquat (DT; DT group). The other 2 diet groups were intraperitoneally injected with 12 mg/kg body weight of DT with either Arg (1%) (DT + Arg) or NCG (0.1%) (DT + NCG). Rat spleen samples were collected for analysis at 48 h after DT injection. Results showed that DT damaged the antioxidant defense in rats compared with the control group ( < 0.05). Compared with the DT group, the DT + Arg and DT + NCG groups manifested improved anti-hydroxyl radical, catalase, and total superoxide dismutase (T-SOD) activities, increased glutathione content ( < 0.05), and decreased malondialdehyde content ( < 0.05). Moreover, compared with the DT group, the DT + Arg and DT + NCG groups enhanced mRNA expression of superoxide dismutase (), glutathione peroxidase 1 (), glutathione reductase (), nuclear factor erythroid 2-related factor 2 (), Kelch-like ECH-associated protein 1(), and mammalian target of rapamycin () ( < 0.05). Both NCG and Arg significantly increased anti-inflammatory cytokine mRNA level but suppressed the pro-inflammatory cytokine mRNA expression under oxidative stress ( < 0.05). In summary, NCG and Arg effectively alleviated oxidative stress, improved the antioxidant capacity and regulated the antioxidant-related signaling molecular expression in rat spleen. N-carbamylglutamate and Arg reduced the inflammation in the spleen by mediating the gene expression of anti-inflammatory and pro-inflammatory cytokines and transforming growth factor-β ().
本研究评估了精氨酸（Arg）或N-氨甲酰谷氨酸（NCG）对氧化应激状态下大鼠脾脏炎症、抗氧化特性及抗氧化相关基因表达的影响。将52只大鼠随机分为4个处理组，每组13个重复。给大鼠饲喂基础日粮（BD）或添加了Arg或NCG的BD，持续30天。在第28天，一半饲喂BD的大鼠腹腔注射无菌生理盐水（对照组），另一半注射12 mg/kg体重的敌草快（DT；DT组）。另外2个日粮组腹腔注射12 mg/kg体重的DT，同时分别添加Arg（1%）（DT + Arg）或NCG（0.1%）（DT + NCG）。在注射DT后48小时采集大鼠脾脏样本进行分析。结果显示，与对照组相比，DT损害了大鼠的抗氧化防御能力（P < 0.05）。与DT组相比，DT + Arg组和DT + NCG组的抗羟自由基、过氧化氢酶和总超氧化物歧化酶（T-SOD）活性提高，谷胱甘肽含量增加（P < 0.05），丙二醛含量降低（P < 0.05）。此外，与DT组相比，DT + Arg组和DT + NCG组超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶1（GPx1）、谷胱甘肽还原酶（GR）、核因子红细胞2相关因子2（Nrf2）、 Kelch样ECH相关蛋白1（Keap1）和雷帕霉素靶蛋白（mTOR）的mRNA表达增强（P < 0.05）。在氧化应激状态下，NCG和Arg均显著提高抗炎细胞因子mRNA水平，但抑制促炎细胞因子mRNA表达（P < 0.05）。综上所述，NCG和Arg有效减轻了氧化应激，提高了抗氧化能力，并调节了大鼠脾脏中抗氧化相关信号分子的表达。N-氨甲酰谷氨酸和精氨酸通过介导抗炎和促炎细胞因子以及转化生长因子-β（TGF-β）的基因表达减轻了脾脏炎症。
