Unité de Chimie Environnementale et Interactions sur le Vivant (UCEIV EA 4492), Université du Littoral Côte d'Opale, 59140, Dunkerque, France.
Environ Sci Pollut Res Int. 2018 Apr;25(12):12177-12182. doi: 10.1007/s11356-017-1164-y. Epub 2018 Feb 1.
In a search for indigenous soil saprotrophic fungi for bioremediation purposes, Fusarium solani, a saprotrophic fungus belonging to the phylum Ascomycota, was isolated from a fossil carbon contaminated soil. The effect of the carbon source, glucose or olive oil, was investigated in vitro on the biomass produced by F. solani and on the degradation of benzo[a]pyrene (BaP) in mineral medium. After only 12 days of incubation, BaP degradation by F. solani was higher (37.4%) with olive oil used as the carbon source than the one obtained with glucose (4.2%). Catalase activity increased in the presence of olive oil (3.4 μkat mg protein) in comparison with glucose (2.1 μkat mg protein). When olive oil was used as the carbon source, BaP degradation increased up to 76.0% in the presence of a specific catalase inhibitor, 3-Amino-1,2,4-triazole (2 mM). This metabolic engineering strategy based both on the use of olive oil as carbon source (cultivation strategy) and on the blocking of the catalase activity could be an innovative and promising approach for fungal biodegradation of BaP and consequently for bioremediation of soil contaminated with polycyclic aromatic hydrocarbons.
为了进行生物修复目的，寻找本土土壤腐生真菌，从污染化石碳的土壤中分离出了属于子囊菌门的腐生真菌茄病镰刀菌（Fusarium solani）。在体外研究了碳源葡萄糖或橄榄油对 F. solani 产生的生物量和在矿物培养基中降解苯并[a]芘（BaP）的影响。在 12 天的孵育后，以橄榄油作为碳源时，F. solani 对 BaP 的降解率（37.4%）高于以葡萄糖作为碳源时的降解率（4.2%）。与葡萄糖（2.1 μkat mg 蛋白）相比，橄榄油存在时过氧化氢酶活性增加（3.4 μkat mg 蛋白）。当使用橄榄油作为碳源时，在特定的过氧化氢酶抑制剂 3-氨基-1,2,4-三唑（2 mM）存在的情况下，BaP 的降解增加至 76.0%。这种基于使用橄榄油作为碳源的代谢工程策略（培养策略）和阻断过氧化氢酶活性的策略可能是真菌生物降解 BaP 并因此修复多环芳烃污染土壤的创新和有前途的方法。
