Newton Ana Paula Negrelo, Cadena Silvia Maria S C, Rocha Maria Eliane Merlin, Carnieri Eva Gunilla Skäre, Martinelli de Oliveira Maria Benigna
Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba, C.P. 19046, CEP 81531-990, Curitiba, Paraná, Brazil.
Toxicol Lett. 2005 Dec 30;160(1):49-59. doi: 10.1016/j.toxlet.2005.06.004. Epub 2005 Jul 14.
Bisphenols are a class of compounds that exhibit a broad spectrum of antimicrobial activity. One of the most widely used member of this group is triclosan (TRN). TRN is a synthetic, non-ionic, broad-spectrum antimicrobial agent, which is incorporated into several products, including hand soaps and detergents and those of skin care and oral hygiene. The effects of TRN on mitochondrial respiratory parameters and the inner mitochondrial membrane potential (DeltaPsi) are described. That of TRN (up to 60 nmol mg(-1) protein) on isolated liver mitochondria decreased oxygen consumption of state 3 respiration, as well as DeltaPsi, but increased oxygen consumption of state 4 respiration, characteristic of an uncoupler effect. Analysis of segments of the respiratory chain suggested that the TRN inhibition site is located between complexes II and III. Mitochondrial swelling, energized or driven by the K+ diffusion potential using valinomycin, was also inhibited by TRN, the former being completely inhibited at concentrations greater than 10 nmol TRN mg(-1) protein, suggesting that it is also able to interfere with fluidity of the inner mitochondrial membrane. These results suggest that, besides its antibacterial effect, TRN can also impair the mitochondrial function of animal cells.
双酚是一类具有广泛抗菌活性的化合物。该类中使用最广泛的成员之一是三氯生（TRN）。TRN是一种合成的非离子型广谱抗菌剂，被添加到多种产品中，包括洗手液、洗涤剂以及护肤品和口腔卫生用品。本文描述了TRN对线粒体呼吸参数和线粒体内膜电位（ΔΨ）的影响。TRN（高达60 nmol mg⁻¹蛋白质）对分离的肝线粒体的作用降低了状态3呼吸的氧气消耗以及ΔΨ，但增加了状态4呼吸的氧气消耗，这是解偶联剂效应的特征。对呼吸链片段的分析表明，TRN的抑制位点位于复合物II和III之间。使用缬氨霉素由K⁺扩散电位驱动或激发的线粒体肿胀也受到TRN的抑制，前者在TRN浓度大于10 nmol mg⁻¹蛋白质时被完全抑制，这表明它也能够干扰线粒体内膜的流动性。这些结果表明，除了其抗菌作用外，TRN还会损害动物细胞的线粒体功能。
