Elghait Amal T Abou, Mostafa Tarek M, Gameaa Fatma K, Mohammed Gamal K, Meligy Fatma Y, Sayed Manal M
Department of Histology and Cell Biology, Faculty of Medicine, Assiut University, Assiut, Egypt.
Department of Histology and Cell Biology, Sphinx University, New Assiut City, Egypt.
Anat Cell Biol. 2022 Sep 30;55(3):341-355. doi: 10.5115/acb.22.013. Epub 2022 Aug 26.
As a synthetic analog of codeine, tramadol is often prescribed to treat mild to moderate pains. This study was designed to estimate and compare the histological effect of tramadol on testes of both juvenile and adult male albino mice. A total number of 40 healthy male albino mice were classified into two main groups as follows: group I (juvenile group, includes 20 mice aged three weeks) subdivided equally into group Ia (control group received isotonic saline) and group Ib (tramadol-treated group received 40 mg/kg/d tramadol orally for 30 days); group II (adult group, includes 20 mice aged two months) subdivided equally into group IIa (control group received isotonic saline) and group IIb (tramadol-treated group). Juvenile and adult tramadol-treated groups showed numerous testicular changes, including blood vessels congestion, widening of intercellular spaces, vacuolization in interstitial tissues, luminal germ cells exfoliation, and increased expression of caspase-3 that indicated cellular apoptosis. In the ultrastructural examination, spermatogenic cells degenerated with the frequent appearance of apoptotic cells. Sertoli cells showed vacuolations, large lipid droplets, and disrupted intercellular cell junctions. These observed testicular changes were markedly observed in the juvenile group. Testicular abnormalities and apoptotic changes can be caused by tramadol administration. These abnormalities are more common in juvenile mice.
曲马多作为可待因的合成类似物，常用于治疗轻至中度疼痛。本研究旨在评估和比较曲马多对幼年和成年雄性白化小鼠睾丸的组织学影响。总共40只健康雄性白化小鼠被分为两个主要组，如下：第一组（幼年组，包括20只三周龄小鼠）再平均分为Ia组（对照组接受等渗盐水）和Ib组（曲马多治疗组口服40mg/kg/d曲马多，持续30天）；第二组（成年组，包括20只两个月龄小鼠）再平均分为IIa组（对照组接受等渗盐水）和IIb组（曲马多治疗组）。幼年和成年曲马多治疗组均出现大量睾丸变化，包括血管充血、细胞间隙增宽、间质组织空泡化、管腔生殖细胞脱落以及表明细胞凋亡的半胱天冬酶-3表达增加。在超微结构检查中，生精细胞退化，凋亡细胞频繁出现。支持细胞出现空泡化、大脂滴以及细胞间连接破坏。这些观察到的睾丸变化在幼年组中更为明显。曲马多给药可导致睾丸异常和凋亡变化。这些异常在幼年小鼠中更为常见。
