Survase Shrikant A, Saudagar Parag S, Singhal Rekha S
Food and Fermentation Technology Department, Institute of Chemical Technology, University of Mumbai, Matunga, Mumbai 400 019, India.
Bioresour Technol. 2006 May;97(8):989-93. doi: 10.1016/j.biortech.2005.04.037. Epub 2005 Jul 18.
Scleroglucan, a neutral homopolysaccaride consisting of a linear chain of beta-D-(1-3)-glucopyranosyl and beta-D-(1-6)-glucopyranosyl groups, was produced by pure culture fermentation from Sclerotium rolfsii MTCC 2156 by submerged culture. Fermentation process was optimized in two steps. In the first step, one-factor-at-a-time method was used to investigate the effects of medium constituents such as carbon and nitrogen sources. In the second step, concentration of medium components was optimized using an L16-orthogonal array method. In all, 10 different carbon sources and eight different nitrogen sources were evaluated. Maximum yield of 16.58 g/l was obtained in a medium containing sucrose as a carbon source and sodium nitrate and yeast extract as nitrogen source.
硬葡聚糖是一种中性同多糖，由β-D-(1-3)-吡喃葡萄糖基和β-D-(1-6)-吡喃葡萄糖基的线性链组成，通过深层培养从齐整小核菌MTCC 2156纯培养发酵产生。发酵过程分两步进行优化。第一步，采用一次单因素法研究碳源和氮源等培养基成分的影响。第二步，使用L16正交表法优化培养基成分的浓度。总共评估了10种不同的碳源和8种不同的氮源。在以蔗糖作为碳源、硝酸钠和酵母提取物作为氮源的培养基中获得了16.58 g/l的最大产量。
