Cowley Siobhán C, Hamilton Elizabeth, Frelinger Jeffrey A, Su Jie, Forman James, Elkins Karen L
Laboratory of Mycobacterial Diseases and Cellular Immunology, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Rockville, MD 20852, USA.
J Exp Med. 2005 Jul 18;202(2):309-19. doi: 10.1084/jem.20050569.
Memory T cells, including the well-known CD4(+) and CD8(+) T cells, are central components of the acquired immune system and are the basis for successful vaccination. After infection, CD4(+) and CD8(+) T cells expand into effector cells, and then differentiate into long-lived memory cells. We show that a rare population of CD4(-)CD8(-)CD3(+)alphabeta(+)gammadelta(-)NK1.1(-) T cells has similar functions. These cells potently and specifically inhibit the growth of the intracellular bacteria Mycobacterium tuberculosis (M. tb.) or Francisella tularensis Live Vaccine Strain (LVS) in macrophages in vitro, promote survival of mice infected with these organisms in vivo, and adoptively transfer immunity to F. tularensis LVS. Furthermore, these cells expand in the spleens of mice infected with M. tb. or F. tularensis LVS, and then acquire a memory cell phenotype. Thus, CD4(-)CD8(-) T cells have a role in the control of intracellular infection and may contribute to successful vaccination.
记忆性T细胞，包括广为人知的CD4(+)和CD8(+) T细胞，是获得性免疫系统的核心组成部分，也是成功接种疫苗的基础。感染后，CD4(+)和CD8(+) T细胞会扩增为效应细胞，然后分化为长寿的记忆细胞。我们发现，一类罕见的CD4(-)CD8(-)CD3(+)αβ(+)γδ(-)NK1.1(-) T细胞具有类似的功能。这些细胞在体外能有效且特异性地抑制巨噬细胞内的胞内菌结核分枝杆菌（M. tb.）或土拉弗朗西斯菌活疫苗株（LVS）的生长，在体内能促进感染这些病原体的小鼠存活，并能将对土拉弗朗西斯菌LVS的免疫力进行过继性转移。此外，这些细胞在感染结核分枝杆菌或土拉弗朗西斯菌LVS小鼠的脾脏中会扩增，然后获得记忆细胞表型。因此，CD4(-)CD8(-) T细胞在控制胞内感染中发挥作用，可能有助于成功接种疫苗。
