Wang Jingxue, Chen Zhenlang, Du Jianzhong, Sun Yi, Liang Aihua
The Agri-Biotechnology Research Centre of Shanxi Province, Taiyuan 030031, P.R. China.
Plant Cell Rep. 2005 Nov;24(9):549-55. doi: 10.1007/s00299-005-0967-3. Epub 2005 Jul 19.
Transgenic plants with introduced pest-resistant gene offer an efficient alternative insect control. The novel insect-resistant gene combination, chitinase(chi) and BmkIT(Bmk), containing an insect-specific chitinase gene and a scorpion insect toxin gene was introduced into Brassica napus cultivar via Agrobacterium-mediated transformation. Fifty-seven regenerated plantlets with kanamycin-resistance were obtained. Transgenic plants were verified by Southern blot analysis. Enzyme-linked immunosorbent assay (ELISA) and bioassay of artificial inoculation with diamondback moth (Plutella maculipenis) (DBM) larvae indicated that some of the transgenic plants were high-level expression for both chitinase and scorpion toxin proteins and performed high resistance against the tested pest infestation. The genetic analysis of T(1) progeny confirmed that the inheritance of introduced genes followed the Mendelian rules.
导入抗虫基因的转基因植物为害虫防治提供了一种有效的替代方法。通过农杆菌介导的转化，将含有昆虫特异性几丁质酶基因和蝎昆虫毒素基因的新型抗虫基因组合几丁质酶（chi）和BmkIT（Bmk）导入甘蓝型油菜品种。获得了57株具有卡那霉素抗性的再生植株。通过Southern杂交分析对转基因植株进行了验证。酶联免疫吸附测定（ELISA）和小菜蛾幼虫人工接种生物测定表明，一些转基因植株中几丁质酶和蝎毒素蛋白均高水平表达，对受试害虫侵染表现出高抗性。T(1)代子代的遗传分析证实，导入基因的遗传遵循孟德尔规律。
