Wang W C, Menon G, Hansen G
Syngenta Biotechnology Inc., 3054 Cornwallis Road, Research Triangle Park, NC 27709, USA.
Plant Cell Rep. 2003 Nov;22(4):274-81. doi: 10.1007/s00299-003-0691-9. Epub 2003 Aug 29.
We report here an in planta method to produce transgenic Brassica napus plants. The procedure included Agrobacterium-mediated inoculation of plants at various development stages along with a vacuum infiltration step. The flowering stage appeared to be the most receptive stage for transformation and production of transgenic plants. In some cases, the flowering stage was induced either by cold treatment or by high density planting. Molecular and genetic analysis revealed that single and multiple copy events were generated and that the transgenes were transmitted to the T1 and T2 progeny in a Mendelian fashion.
我们在此报告一种在植物体内生产转基因油菜植株的方法。该程序包括在不同发育阶段通过农杆菌介导接种植株以及真空渗透步骤。开花期似乎是转化和生产转基因植株最易接受的阶段。在某些情况下，通过冷处理或高密度种植诱导开花期。分子和遗传分析表明，产生了单拷贝和多拷贝事件，并且转基因以孟德尔方式传递给T1和T2代后代。
