Kittel Agnes, Kiss Anna L, Müllner Nándor, Matkó Ida, Sperlágh Beáta
Department of Pharmacology, Hungarian Academy of Sciences, Institute of Experimental Medicine, P.O. Box 67, 1450 Budapest, Hungary.
Histochem Cell Biol. 2005 Jul;124(1):51-9. doi: 10.1007/s00418-005-0018-8. Epub 2005 Jul 19.
Pathological circumstances like inflammation or ischemic insult facilitate the release of adenine nucleotides from several types of cells. These extracellular nucleotides are rapidly converted to adenosine by ectonucleotidases, mainly ectonucleoside triphosphate diphosphohydrolase1 (NTPDase1/CD39) and CD73. NTPDase1/CD39 can interact with caveolins, structural proteins of signal-transducing microdomains termed caveolae. Caveolins are thought to have physiological roles in heart ageing and cardiac diseases. The aim of this study was to investigate the expression of NTPDase1 together with caveolins in chronic human cardiovascular diseases and elucidate their role in human heart. The HPLC analysis showed significant increase in ATPase activity in pathological samples from patients with ischemic heart disease. Immunostaining also showed alterations in the expression and distribution of NTPDase1. Caveolin-1 and caveolin-2 expression was much alike in control and pathological cases, while expression of caveolin-3 was lower in pathological samples. Changes in the expression of NTPDase1 and caveolins seem to be independent of human cardiovascular disease.
诸如炎症或缺血性损伤等病理情况会促使几种类型的细胞释放腺嘌呤核苷酸。这些细胞外核苷酸会被外核苷酸酶迅速转化为腺苷，主要是外核苷三磷酸二磷酸水解酶1（NTPDase1/CD39）和CD73。NTPDase1/CD39可与小窝蛋白相互作用，小窝蛋白是称为小窝的信号转导微结构域的结构蛋白。小窝蛋白被认为在心脏衰老和心脏疾病中具有生理作用。本研究的目的是调查慢性人类心血管疾病中NTPDase1与小窝蛋白的表达情况，并阐明它们在人类心脏中的作用。高效液相色谱分析显示，缺血性心脏病患者病理样本中的ATP酶活性显著增加。免疫染色还显示NTPDase1的表达和分布发生了改变。小窝蛋白-1和小窝蛋白-2在对照和病理病例中的表达非常相似，而病理样本中小窝蛋白-3的表达较低。NTPDase1和小窝蛋白表达的变化似乎与人类心血管疾病无关。
