Maeyama Ryo, Kwon Il Keun, Mizunoe Yoshimitsu, Anderson James M, Tanaka Masao, Matsuda Takehisa
Division of Biomedical Engineering, Faculty of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
J Biomed Mater Res A. 2005 Oct 1;75(1):146-55. doi: 10.1002/jbm.a.30346.
We developed a novel bactericidal surface based on a catechin-loaded surface-erodible polymer. (-)-Epigallocatechin-3-gallate (EGCg), which is the main constituent of tea catechins, showed a dose-dependent inhibitory effect on Escherichia coli biofilm formation and a dose-dependent enhanced destructive effect on biofilm. EGCg-immobilized surfaces were prepared by photopolymerization of liquid biodegradable polyesters. The releasing rate was enhanced with an increase in surface-erosion rate of photocured polymers. Polymers with high releasing capacity dose-dependently reduced biofilm formation on the surfaces. The confocal laser scanning microscopic and scanning electron microscopic observations revealed that EGCg induced biofilm-destructing activities, which include bacterial membrane damage, degradation of exopolysaccharides, and detachment of colonized cells. From these results, potential advantages of the clinical use of catechin-loaded polymer-coated implants or catheters are discussed in terms of a reduced occurrence of biomaterial-centered infections without substantial toxicity or adverse effects.
我们开发了一种基于负载儿茶素的表面可蚀聚合物的新型杀菌表面。（-）-表没食子儿茶素-3-没食子酸酯（EGCg）是茶儿茶素的主要成分，对大肠杆菌生物膜形成呈现剂量依赖性抑制作用，对生物膜具有剂量依赖性增强的破坏作用。通过液体可生物降解聚酯的光聚合制备了固定有EGCg的表面。随着光固化聚合物表面侵蚀速率的增加，释放速率提高。具有高释放能力的聚合物剂量依赖性地减少了表面生物膜的形成。共聚焦激光扫描显微镜和扫描电子显微镜观察表明，EGCg诱导生物膜破坏活性，包括细菌膜损伤、胞外多糖降解和定植细胞脱落。基于这些结果，就减少以生物材料为中心的感染发生率且无实质性毒性或不良反应而言，讨论了负载儿茶素的聚合物涂层植入物或导管临床应用的潜在优势。
