Chen Li, Wang Jian-min, Xu Xiao-ping, Li Yao, Xu Hong, Ju Xiao-ping, Ni Xiong, Yang Jian-min, Xu Yan-qun
Department of Hematology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
Zhonghua Yi Xue Za Zhi. 2005 Apr 27;85(16):1089-92.
To investigate the gene expression of acute leukemia so as to study the pathogenesis of leukemia.
Five ml of bone marrow was collected from 22 patients with leukemia, 15 males and 7 females, aged 15-86, 17 with acute myelocytic leukemia, 4 with acute lymphocytic leukemia, and 1 with AHL. Mononuclear cells were isolated. Total RNA was extracted and mRNA was purified. DNA microarray technique with 12 848 genes was used to analyze the gene expression profiles.
The predicted 45 genes were enormously expressed in 21 patients with acute leukemia, which were consistent with the ALL and AML classification standards reported by Golub and others. 7 genes which were overexpressed in multiple-resistant cell line K562-n/VCR were also overexpressed in 6 cases of refractory acute leukemia. Further analysis showed that 31 genes were upregulated in AML (M(4)-M(6)) and ALL but downregulated in AML (M(1)-M(3)).
This results support the standard Golub has put forward. The reason that adult patients with ALL and subtypes of AML (M(4)-M(6)) have poorer prognosis in comparison with AMLM(1)-M(3) is attributed to alterations in gene expression. Primary drug resistance may be the major characteristics of refractory leukemia. Analysis of gene expression profile in acute leukemia is significant for classification and prognosis.
研究急性白血病的基因表达情况，以探讨白血病的发病机制。
收集22例白血病患者的骨髓5ml，其中男性15例，女性7例，年龄15 - 86岁，急性髓细胞白血病17例，急性淋巴细胞白血病4例，急性混合细胞白血病1例。分离单个核细胞，提取总RNA并纯化mRNA。采用含12848个基因的DNA微阵列技术分析基因表达谱。
预测的45个基因在21例急性白血病患者中高表达，与Golub等人报道的ALL和AML分类标准一致。在多药耐药细胞系K562 - n/VCR中高表达的7个基因，在6例难治性急性白血病中也高表达。进一步分析显示，31个基因在AML（M(4)-M(6)）和ALL中上调，但在AML（M(1)-M(3)）中下调。
本研究结果支持Golub提出的标准。成人ALL和AML亚型（M(4)-M(6)）预后较AML（M(1)-M(3)）差的原因归因于基因表达的改变。原发性耐药可能是难治性白血病的主要特征。分析急性白血病的基因表达谱对分类和预后判断具有重要意义。
