Yoshimoto H, Ohmae M, Yamashita I
Center for Gene Science, Hiroshima University, Japan.
Mol Gen Genet. 1992 May;233(1-2):327-30. doi: 10.1007/BF00587597.
We have cloned GAM2, which is required for transcription of STA1, a gene encoding an extracellular glucoamylase in Saccharomyces cerevisiae var. diastaticus. DNA sequence analysis revealed that GAM2 is the same gene as SIN3, known to be a general negative regulator of yeast genes. RNA blot analysis indicated that GAM2/SIN3 also acts as a positive regulator of GAM3/ADR6, which in turn is required for transcription of STA1 and ADH2. These results suggest that GAM2 regulates STA1 expression through transcriptional activation of GAM3 and indicate that GAM2/SIN3 protein is a transcriptional regulator that can play a role in both activation and repression of transcription.
我们已经克隆了GAM2，它是酿酒酵母变种diastaticus中STA1转录所必需的，STA1是一个编码细胞外葡糖淀粉酶的基因。DNA序列分析表明，GAM2与SIN3是同一基因，已知SIN3是酵母基因的一般负调控因子。RNA印迹分析表明，GAM2/SIN3也作为GAM3/ADR6的正调控因子，而GAM3/ADR6又是STA1和ADH2转录所必需的。这些结果表明，GAM2通过GAM3的转录激活来调节STA1的表达，并表明GAM2/SIN3蛋白是一种转录调节因子，可在转录的激活和抑制中发挥作用。
