Hutchison M L, Walters L D, Avery S M, Moore A
Direct Laboratories Research Division, Wolverhampton, West Midlands, UK.
J Appl Microbiol. 2005;99(2):354-62. doi: 10.1111/j.1365-2672.2005.02591.x.
To measure the rates of decline of zoonotic agents introduced into heaps of spent bedding and faecal wastes generated by commercially farmed livestock and managed in a similar way to that of a working farm.
Livestock isolates of Salmonella, pathogenic Listeria, Campylobacter and Escherichia coli O157 were laboratory cultured and used to inoculate 5 m3 heaps of cattle, sheep or pig wastes mixed with bedding materials. Decline of each of the infectious agents was monitored with time as was the temperature inside each heap. Temperatures of >50 degrees C were typically achieved at the core of the heaps. Pathogen decline was rapid, typically <3 days for a 1-log reduction in levels. The longest time that zoonotic agents were isolated from the heaps was 93 days.
Movement of heaps of livestock bedding waste from animal pens to a secondary store, and storing them under conditions conducive for increased temperature is a simple and cost-effective treatment for rapidly lowering levels of zoonotic agents in solid farm wastes.
This study demonstrates a simple and cheap treatment that can be used to help prevent the spread of zoonotic agents through agricultural environments.
测定引入到商业化养殖家畜产生的用过的垫料和粪便堆中的人畜共患病原体的衰减率，这些粪便堆的管理方式与实际运营农场的管理方式类似。
对沙门氏菌、致病性李斯特菌、弯曲杆菌和大肠杆菌O157的家畜分离株进行实验室培养，并用于接种5立方米的牛、羊或猪粪便与垫料混合的堆肥。随着时间的推移监测每种传染源的衰减情况以及每个粪便堆内部的温度。粪便堆核心部位的温度通常能达到50摄氏度以上。病原体衰减迅速，水平降低1个对数通常不到3天。从粪便堆中分离出人畜共患病原体的最长时间为93天。
将家畜垫料粪便堆从畜栏转移到二级储存处，并在有利于升温的条件下储存，是一种简单且经济高效的处理方法，可快速降低固体农场废弃物中的人畜共患病原体水平。
本研究展示了一种简单且廉价的处理方法，可用于帮助预防人畜共患病原体在农业环境中的传播。
