Hutchison M L, Walters L D, Moore A, Crookes K M, Avery S M
Microbiological Research Division, Direct Laboratories, Ltd., Woodthorne, Wergs Rd., Wolverhampton WV6 8TQ, United Kingdom.
Appl Environ Microbiol. 2004 Sep;70(9):5111-8. doi: 10.1128/AEM.70.9.5111-5118.2004.
In response to reports that the contamination of food can occur during the on-farm primary phase of food production, we report data that describes a possible cost-effective intervention measure. The effect of time before soil incorporation of livestock wastes spread to land on the rate of decline of zoonotic agents present in the waste was investigated. Fresh livestock wastes were inoculated with laboratory-cultured Salmonella, Listeria, and Campylobacter spp. and Escherichia coli O157 before they were spread onto soil. Incorporation of the spread wastes was either immediate, delayed for 1 week, or did not occur at all. Bacterial decline was monitored over time and found to be significantly more rapid for all waste types when they were left on the soil surface. There were no significant differences in initial bacterial decline rates when wastes were spread in summer or winter. Our results indicate that not incorporating contaminated livestock wastes into soil is a potential intervention measure that may help to limit the spread of zoonotic agents further up the food chain. The implications of these findings are discussed in relation to current advice for livestock waste disposal.
针对有关食品污染可能发生在农场食品生产初级阶段的报道，我们报告了描述一种可能具有成本效益的干预措施的数据。研究了将牲畜粪便铺撒到土地上后至混入土壤之前的时间对粪便中存在的人畜共患病原体衰减率的影响。在将新鲜牲畜粪便铺撒到土壤之前，先接种实验室培养的沙门氏菌、李斯特菌、弯曲杆菌属以及大肠杆菌O157。铺撒后的粪便要么立即混入土壤，要么延迟1周混入，要么根本不混入。随着时间的推移监测细菌衰减情况，发现所有类型的粪便留在土壤表面时，细菌衰减明显更快。粪便在夏季或冬季铺撒时，初始细菌衰减率没有显著差异。我们的结果表明，不将受污染的牲畜粪便混入土壤是一种潜在的干预措施，可能有助于限制人畜共患病原体在食物链中进一步传播。结合当前关于牲畜粪便处理的建议，讨论了这些发现的意义。
