Hutchison M L, Walters L D, Moore A, Avery S M
Microbiology Research, Direct Laboratory Services Ltd, Wergs Road, Wolverhampton, UK.
J Appl Microbiol. 2005;99(1):58-65. doi: 10.1111/j.1365-2672.2005.02585.x.
To measure the decline rates of zoonotic agents introduced into liquid livestock wastes in on-farm storage tanks.
Salmonella spp., Escherichia coli O157, Campylobacter jejuni, Listeria monocytogenes and Cryptosporidium parvum, propagated in laboratory-controlled conditions, were inoculated into 35,000-l volumes of fresh livestock wastes (pig slurries, cattle slurries and dirty waters). D-values for bacteria were six to 44 days, and for C. parvum were 133 to 345 days. Campylobacter jejuni declined significantly more rapidly than the other bacterial pathogens, while E. coli O157 declined significantly more slowly. On average, bacterial declines were not affected by the season of waste deposition and storage or by the dry matter content of the wastes, but were more rapid in dirty waters than in pig slurries. The physiciochemical composition of wastes in each category varied significantly.
Zoonotic agents can survive for several months during storage of liquid livestock wastes. Livestock wastes should be batch-stored and not subjected to continuous additions.
This study indicates that batches of liquid livestock waste, if contaminated with bacterial pathogens, should be stored for 6 months to reduce contamination levels. Alternative strategies for reducing C. parvum levels in liquid livestock wastes should be explored.
测定引入农场储存罐中液态家畜粪便中的人畜共患病原体的衰减率。
在实验室控制条件下繁殖的沙门氏菌属、大肠杆菌O157、空肠弯曲菌、单核细胞增生李斯特菌和微小隐孢子虫，被接种到35000升新鲜家畜粪便（猪粪、牛粪和污水）中。细菌的D值为6至44天，微小隐孢子虫的D值为133至345天。空肠弯曲菌的衰减明显比其他细菌病原体更快，而大肠杆菌O157的衰减明显更慢。平均而言，细菌的衰减不受粪便沉积和储存季节或粪便干物质含量的影响，但在污水中比在猪粪中衰减更快。每类粪便的物理化学组成差异显著。
人畜共患病原体在液态家畜粪便储存期间可存活数月。家畜粪便应分批储存，不应持续添加。
本研究表明，如果液态家畜粪便被细菌病原体污染，应储存6个月以降低污染水平。应探索降低液态家畜粪便中微小隐孢子虫水平的替代策略。
