Schriek Gernot, Oppitz Matthias, Busch Christian, Just Lothar, Drews Ulrich
Institute of Anatomy, Department of Experimental Embryology, University of Tübingen, Tübingen, Germany.
Melanoma Res. 2005 Aug;15(4):225-34. doi: 10.1097/00008390-200508000-00001.
Melanocytes are derived from the neural crest. We questioned whether the migratory mechanism during the invasive growth of melanoma cells is the same as that in neural crest cell migration. We transplanted human SK-Mel 28 melanoma cells into the neural tube of the chick embryo stage 11-13 and, after up to 6 days of total incubation, traced the cells by immunohistochemistry in serial paraffin sections. SK-Mel 28 cells were integrated into the host neural crest and were found in the roof plate of the neural tube, along the medial neural crest cell pathway, in the sclerotome and, finally, in developing sympathetic ganglia. At stage 21, massive segmental emigration between myotome and disintegrating dermatome was observed at the level of the upper limb bud. The melanoma cells, in contrast with the chick neural crest cells, were HNK-1-negative. They retained the premelanosome epitope HMB-45. For definite identification and exclusion of fusion with chick embryo cells, in situ hybridization with the human-specific Alu sequence was performed. The results showed that human SK-Mel 28 melanoma cells were capable of resuming neural crest cell migration in the embryo.
黑素细胞起源于神经嵴。我们质疑黑色素瘤细胞侵袭性生长过程中的迁移机制是否与神经嵴细胞迁移的机制相同。我们将人SK-Mel 28黑色素瘤细胞移植到第11 - 13期鸡胚的神经管中，在总共培养6天后，通过免疫组织化学在连续石蜡切片中追踪这些细胞。SK-Mel 28细胞整合到宿主神经嵴中，并在神经管的顶板、沿内侧神经嵴细胞路径、在体节中，最终在发育中的交感神经节中被发现。在第21期，在上肢芽水平观察到肌节和正在解体的皮节之间有大量节段性迁移。与鸡神经嵴细胞相比，黑色素瘤细胞HNK-1阴性。它们保留了前黑素体抗原决定簇HMB-45。为了明确鉴定并排除与鸡胚细胞融合，进行了与人特异性Alu序列的原位杂交。结果表明，人SK-Mel 28黑色素瘤细胞能够在胚胎中恢复神经嵴细胞迁移。
