Nair S, Lee Y H, Rousseau E, Cam M, Tataranni P A, Baier L J, Bogardus C, Permana P A
Obesity and Diabetes Clinical Research Section, Phoenix Epidemiology and Clinical Research Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Phoenix, AZ 85016, USA.
Diabetologia. 2005 Sep;48(9):1784-8. doi: 10.1007/s00125-005-1868-2. Epub 2005 Jul 21.
AIMS/HYPOTHESIS: The specific contributions made by the various cell types in adipose tissue to obesity, particularly obesity-related inflammation, need to be clarified. The aim of this study was to elucidate the potential role of adipocyte precursor cells (preadipocytes/stromal vascular cells [SVC]).
We performed Affymetrix oligonucleotide microarray expression profiling of cultured abdominal subcutaneous preadipocytes/SVC isolated from the adipose tissue of 14 non-obese (BMI 25+/-4 kg/m2) and 14 obese (55+/-8 kg/m2) non-diabetic Pima Indian subjects. Quantitative real-time PCR (RT-PCR) was used to verify the differential expression of several genes in an independent group of subjects.
We identified 218 differentially expressed genes with p values less than 0.01. Microarray expression profiling revealed that the expression of inflammation-related genes was significantly upregulated in preadipocytes/SVC of obese individuals. Quantitative RT-PCR confirmed the upregulation of IL8, CTSS, ITGB2, HLA-DRA, CD53, PLA2G7 and MMP9 in preadipocytes/SVC of obese subjects.
CONCLUSIONS/INTERPRETATION: The upregulation of inflammation-related genes in preadipocytes/SVC of obese subjects may increase the recruitment of immune cells into adipose tissue and may also result in changes in the extracellular matrix (tissue remodelling) to accommodate adipose tissue expansion in obesity.
目的/假设：脂肪组织中各种细胞类型对肥胖，尤其是与肥胖相关的炎症的具体作用尚需阐明。本研究的目的是阐明脂肪细胞前体细胞（前脂肪细胞/基质血管细胞[SVC]）的潜在作用。
我们对从14名非肥胖（体重指数25±4 kg/m²）和14名肥胖（55±8 kg/m²）的非糖尿病皮马印第安人受试者的脂肪组织中分离出的腹部皮下前脂肪细胞/SVC进行了Affymetrix寡核苷酸微阵列表达谱分析。采用定量实时PCR（RT-PCR）在另一组独立受试者中验证了几个基因的差异表达。
我们鉴定出218个差异表达基因，其p值小于0.01。微阵列表达谱显示，肥胖个体的前脂肪细胞/SVC中炎症相关基因的表达显著上调。定量RT-PCR证实肥胖受试者的前脂肪细胞/SVC中IL8、CTSS、ITGB2、HLA-DRA、CD53、PLA2G7和MMP9表达上调。
结论/解读：肥胖受试者前脂肪细胞/SVC中炎症相关基因的上调可能会增加免疫细胞向脂肪组织的募集，也可能导致细胞外基质的变化（组织重塑），以适应肥胖状态下脂肪组织的扩张。
