Sun Ying-Ling, Jan Meei-Ling, Kao Pan-Fu, Fan Kuo-Hsien, Hsu Hui-Ting, Chang Wen-Chen, Shen Young-Chi, Lee Te-Wei
Institute of Nuclear Energy Research, Lung-Tan, Taiwan.
Kaohsiung J Med Sci. 2005 Jun;21(6):258-66. doi: 10.1016/S1607-551X(09)70198-6.
The Institute of Nuclear Energy Research of Taiwan has developed a dynamic coincidence detection device for positron emitted radiotracer pharmacodynamic study in small mice models. In this study, we set up an experimental paradigm by determining [fluorine-18]-2-deoxy-2-fluoro-D-glucose ([18F]FDG) dynamic uptake in tumors and inflammations in nude mice as the foundation for future applications in therapy development. Histopathology and micro-autoradiography of these tumors and inflammations were obtained for confirmation. Dynamic coincidence planar images of six tumors and two inflammations in nude mice were acquired over 4 hours immediately after injection of 25.9 MBq of [18F]FDG into the right thigh of each animal. After image reconstruction, the lesion-to-background ratios were calculated in regions of interest over the lesion and contralateral thigh to determine the equilibrium status of the radiotracer. All mice were sacrificed for histopathologic examination and six of the mice were examined with micro-autoradiography. [18F]FDG uptake in tumors and inflammations both reached equilibrium about 3 hours after injection. At equilibrium, [18F]FDG uptake into tumors was two to four times higher than the background. Uptake into the 4-day and 8-day inflammations was 2.3 and 5.5 times higher than the background, respectively. Histopathology showed macrophage and neutrophil infiltration around the tumors and in the inflammations. Micro-autoradiography showed dense silver grains in the granulation tissue surrounding the tumors and inflammations. The preliminary results suggested that dynamic [18F]FDG coincidence planar imaging can help in determining the suitable time for static [18F]FDG imaging in nude mice models. The optimal time for static [18F]FDG positron emission tomography imaging was around 3 hours after injection. The paradigm for determining a dynamic [18F]FDG uptake pattern was demonstrated for future new therapeutic drug experimental use.
台湾核能研究所开发了一种用于在小型小鼠模型中进行正电子发射放射性示踪剂药效学研究的动态符合探测装置。在本研究中，我们通过测定裸鼠肿瘤和炎症中[氟-18]-2-脱氧-2-氟-D-葡萄糖（[18F]FDG）的动态摄取，建立了一种实验范式，作为未来治疗开发应用的基础。获取了这些肿瘤和炎症的组织病理学和微放射自显影片以进行确认。在向每只动物的右大腿注射25.9 MBq的[18F]FDG后，立即在4小时内获取了裸鼠中六个肿瘤和两个炎症的动态符合平面图像。图像重建后，计算病变区域和对侧大腿感兴趣区域的病变与背景比值，以确定放射性示踪剂的平衡状态。所有小鼠均处死后进行组织病理学检查，其中六只小鼠进行了微放射自显影检查。肿瘤和炎症中[18F]FDG的摄取在注射后约3小时均达到平衡。平衡时，肿瘤中[18F]FDG的摄取比背景高两到四倍。4天和8天炎症中的摄取分别比背景高2.3倍和5.5倍。组织病理学显示肿瘤周围和炎症中有巨噬细胞和中性粒细胞浸润。微放射自显影显示肿瘤和炎症周围的肉芽组织中有密集的银颗粒。初步结果表明，动态[18F]FDG符合平面成像有助于确定裸鼠模型中静态[18F]FDG成像的合适时间。静态[18F]FDG正电子发射断层成像的最佳时间是注射后约3小时。确定动态[18F]FDG摄取模式的范式被证明可用于未来新治疗药物的实验使用。
