Wang F N, Suh B Y
Department of Obstetrics and Gynecology, King/Drew Medical Center, Charles R. Drew University of Medicine and Sciences, Los Angeles, California 90059, USA.
Arch Androl. 2005 Jul-Aug;51(4):317-26. doi: 10.1080/014850190922649.
In this study, we arbitrarily classified the morphological changes of sperm under hypo-osmotic condition and compared the results with four different sperm separation techniques. The morphology of classification ranged from a good swelling (SG 3 > SG 2 > SG 1), to non-swelling but reactive (SG 0-R), and completely non-swelling (SG 0). Thirty fresh semen from patients were divided into 4 groups and each processed by washing, swim up, 2-layer column and real time micro-separation system. The prevalent patterns of the swollen sperm in sequence after treatment was SG 0 > SG 1 > SG 3 > SG2 > SG 0-R in fresh semen, and the proportion of SG 0 was almost half of the total % HOS test results. However, SG 3 became the most common swollen form with a significant increase in number with any method of sperm preparation after treatment. The real time technique yielded the highest % rate of SG 3 type of swollen spermatozoa and was the richest in concentration (53+/-3.9%, P < 0.05) as compared with that of swim up (26+/-4.6%), 2-layer (20+/-4.4%), sperm washing (23+/-3.9%), and fresh semen (17+/- 2.8%), respectively. By contrast, 2-layer collected more number of SG0-R (5.3+/-1.2%, P < 0.05) pattern of spermatozoa when compared to real time and swim up. A positive correlation (r = 0.81, P < 0.002) was shown between the % total HOS spermatozoa and total motility of fresh semen but not with morphology. Collectively, an abnormally functional sperm may exist in almost half of a total sperm count from men. It would appear that a better potential fertilization capacity may reside in the more swollen sperm from HOS test than the less swollen sperm and that may be more related with the motility rather than the morphology. The HOS classification and grading system appears valuable in further evaluating sperm quality.
在本研究中，我们对低渗条件下精子的形态变化进行了任意分类，并将结果与四种不同的精子分离技术进行了比较。分类的形态范围从良好肿胀（SG 3 > SG 2 > SG 1）到未肿胀但有反应（SG 0-R），以及完全未肿胀（SG 0）。来自患者的30份新鲜精液被分为4组，每组分别通过洗涤、上游法、双层柱法和实时微分离系统进行处理。新鲜精液经处理后，肿胀精子的普遍模式依次为SG 0 > SG 1 > SG 3 > SG2 > SG 0-R，SG 0的比例几乎占低渗肿胀试验总结果的一半。然而，SG 3成为最常见的肿胀形式，处理后采用任何精子制备方法其数量均显著增加。实时技术产生的SG 3型肿胀精子的百分比最高，浓度最丰富（53±3.9%，P < 0.05），与之相比，上游法为（26±4.6%），双层柱法为（20±4.4%），精子洗涤法为（23±3.9%），新鲜精液为（17±2.8%）。相比之下，与实时法和上游法相比，双层柱法收集到更多数量的SG0-R模式的精子（5.3±1.2%，P < 0.05）。新鲜精液中低渗肿胀精子总数的百分比与总活力呈正相关（r = 0.81，P < 0.002），但与形态无关。总体而言，男性精子总数中几乎一半可能存在功能异常的精子。似乎低渗肿胀试验中肿胀程度较高的精子比肿胀程度较低的精子具有更好的潜在受精能力，这可能与活力而非形态更相关。低渗肿胀分类和分级系统在进一步评估精子质量方面似乎很有价值。
