Melendez Robert F, Kumar Neeru, Maswadi Saher M, Zaslow Kenneth, Glickmank Randolph D
Department of Ophthalmology, University of Texas Health Science Center, San Antonio, 78229, USA.
Am J Ophthalmol. 2005 Jul;140(1):132-4. doi: 10.1016/j.ajo.2004.12.086.
The purpose of this study was to evaluate the toxicity and photodynamic activity of indocyanine green (ICG) and trypan blue (TryB) on cultured human lensepithelial cells (LECs).
Experimental study.
Lens epithelial cell viability was assessed after treatment with ICG and TryB concentrations ranging from 0.025 to 5.0 mg/ml, and exposure to 806 nm diode laser.
At ICG concentrations below 0.5 mg/ml, there was > or =75% cell viability; at higher ICG concentrations there was dose-dependent cytotoxicity in addition to loss of cellular viability due to ICG photosensitization. TryB had little cytotoxicity to the LECs: >80% cells were viable irrespective of the dye concentration or laser treatment.
These data indicate that ICG may have application as a photosensitizer in the selective eradication of residual LECs after cataract surgery to reduce the incidence of posterior capsule opacification.
本研究旨在评估吲哚菁绿（ICG）和台盼蓝（TryB）对培养的人晶状体上皮细胞（LEC）的毒性和光动力活性。
实验研究。
用浓度范围为0.025至5.0mg/ml的ICG和TryB处理细胞，并暴露于806nm二极管激光后，评估晶状体上皮细胞的活力。
ICG浓度低于0.5mg/ml时，细胞活力≥75%；ICG浓度较高时，除了由于ICG光致敏导致细胞活力丧失外，还存在剂量依赖性细胞毒性。TryB对LEC几乎没有细胞毒性：无论染料浓度或激光处理如何，>80%的细胞存活。
这些数据表明，ICG作为一种光敏剂，可能在白内障手术后选择性根除残留LEC以降低后囊膜混浊发生率方面具有应用价值。
