Chang Yi-Sheng, Tseng Shih-Ya, Tseng Sung-Huei, Chen Ying-Ting, Hsiao Jing-Hsing
Department of Ophthalmology, and the Institute of Clinical Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
J Cataract Refract Surg. 2005 Apr;31(4):792-8. doi: 10.1016/j.jcrs.2004.07.020.
To investigate the corneal endothelial cytotoxicity of dyes for capsule staining in cataract surgery.
Department of Ophthalmology and Institute of Clinical Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
Cultured corneal endothelial cells of New Zealand white rabbits were exposed for 1 minute to 1 of the following dyes (various concentrations): indocyanine green (ICG), methylene blue (MB), gentian violet (GV), trypan blue (TB), and fluorescein sodium (FS). The degree of cell damage was determined by in vitro staining with TB and comparison with results in a control group. The effect of longer exposure (up to 10 minutes) to ICG 0.25% was also investigated. Structural changes in corneal endothelial cells after dye exposure were evaluated by light microscopy and transmission electron microscopy (TEM).
Indocyanine green 0.25%, MB 0.20%, GV 0.01%, TB 0.40%, and FS 10% did not induce significant damage to corneal endothelial cells. Significant cytotoxicity was observed with the following or higher dye concentrations: ICG 0.50%, MB 0.50%, and GV 0.10%. Exposure to ICG 0.25% for 1 to 10 minutes showed a trend toward cytotoxicity after 10 minutes. On TEM, corneal endothelial cells that had been exposed to ICG 0.50% showed remarkable organelle swelling and disruption, electron-dense granules, and cell lysis.
One minute of exposure to ICG 0.25%, MB 0.20%, GV 0.01%, TB 0.40%, and FS 10% appeared to be safe as determined by no cytotoxic effects on rabbit corneal endothelial cells in culture.
研究白内障手术中用于囊膜染色的染料对角膜内皮细胞的细胞毒性。
台湾台南国立成功大学医学院眼科与临床医学研究所。
将新西兰白兔培养的角膜内皮细胞暴露于以下染料（不同浓度）1分钟：吲哚菁绿（ICG）、亚甲蓝（MB）、龙胆紫（GV）、台盼蓝（TB）和荧光素钠（FS）。通过用TB进行体外染色并与对照组结果比较来确定细胞损伤程度。还研究了长时间（长达10分钟）暴露于0.25%的ICG的影响。通过光学显微镜和透射电子显微镜（TEM）评估染料暴露后角膜内皮细胞的结构变化。
0.25%的吲哚菁绿、0.20%的亚甲蓝、0.01%的龙胆紫、0.40%的台盼蓝和10%的荧光素钠未对角膜内皮细胞造成明显损伤。在以下或更高染料浓度下观察到明显的细胞毒性：0.50%的ICG、0.50%的MB和0.10%的GV。暴露于0.25%的ICG 1至10分钟，10分钟后显示出细胞毒性趋势。在TEM下，暴露于0.50%的ICG的角膜内皮细胞显示出明显的细胞器肿胀和破坏、电子致密颗粒和细胞溶解。
根据对培养的兔角膜内皮细胞无细胞毒性作用确定，暴露于浓度为0.25%的ICG、0.20%的MB、0.01%的GV、0.40%的TB和10%的FS 1分钟似乎是安全的。
