恒河猴辐射杂种图谱及其与人类基因组的比较分析。

A rhesus macaque radiation hybrid map and comparative analysis with the human genome.

作者信息

Murphy William J, Agarwala Richa, Schäffer Alejandro A, Stephens Robert, Smith Clarence, Crumpler Nicole J, David Victor A, O'Brien Stephen J

机构信息

Basic Research Laboratory, SAIC-Frederick, Inc., Laboratory of Genomic Diversity, National Cancer Institute, Frederick, MD 21702, USA.

出版信息

Genomics. 2005 Oct;86(4):383-95. doi: 10.1016/j.ygeno.2005.05.013.

DOI:10.1016/j.ygeno.2005.05.013

PMID:16039092

Abstract

The genomes of nonhuman primates are powerful references for better understanding the recent evolution of the human genome. Here we compare the order of 802 genomic markers mapped in a rhesus macaque (Macaca mulatta) radiation hybrid panel with the human genome, allowing for nearly complete cross-reference to the human genome at an average resolution of 3.5 Mb. At least 23 large-scale chromosomal rearrangements, mostly inversions, are needed to explain the changes in marker order between human and macaque. Analysis of the breakpoints flanking inverted chromosomal segments and estimation of their duplication divergence dates provide additional evidence implicating segmental duplications as a major mechanism of chromosomal rearrangement in recent primate evolution.

摘要

非人灵长类动物的基因组是更好地理解人类基因组近期进化的有力参考。在此，我们将恒河猴辐射杂种细胞系中定位的802个基因组标记的顺序与人类基因组进行比较，从而能够以平均3.5 Mb的分辨率对人类基因组进行几乎完全的交叉比对。人类与猕猴之间标记顺序的变化至少需要23次大规模染色体重排来解释，其中大部分是倒位。对倒位染色体片段两侧断点的分析以及对其重复分歧日期的估计，为片段重复作为灵长类动物近期进化中染色体重排的主要机制提供了额外证据。