Tsuzuki Wakako
National Food Research Institute, Kannondai 2-1-12, Tsukuba, Ibaraki 305-8642, Japan.
Biosci Biotechnol Biochem. 2005 Jul;69(7):1256-61. doi: 10.1271/bbb.69.1256.
Ten kinds of lipases were examined as biocatalysts for the incorporation of short-chain fatty acids (acetic, propionic, and butyric acids) into triolein in order to produce one kind of reduced-calorie structured lipids. Trans-esterification (acidolysis) was successfully done in n-hexane by several microbial lipases. Among them, lipase from Aspergillus oryzae was used to investigate the effects of incubation time, substrate molar ratio, and water content on acidolysis. Finally, more than 80% of triolein was incorporated by butyric acid (molar ratio of triolein to butyric acid, 1:10) in the dried n-hexane at 52 degrees C for 72 h. More than 90% of the products was monosubstituent, which was esterified with this short chain fatty acid at the 1-position of the glycerol moiety of triolein. These results suggest that A. oryzae lipase would be a powerful biocatalyst for the synthesis of low caloric oil, such as triacylglycerol containing a mixture of long- and short-chain aliphatic acids.
为了生产一种低热量的结构化脂质，研究了十种脂肪酶作为生物催化剂用于将短链脂肪酸（乙酸、丙酸和丁酸）掺入三油酸甘油酯中。几种微生物脂肪酶成功地在正己烷中进行了酯交换反应（酸解反应）。其中，使用米曲霉脂肪酶研究了孵育时间、底物摩尔比和含水量对酸解反应的影响。最后，在52℃下于干燥的正己烷中反应72小时，丁酸（三油酸甘油酯与丁酸的摩尔比为1:10）掺入三油酸甘油酯的比例超过80%。超过90%的产物是单取代产物，即在三油酸甘油酯甘油部分的1位用这种短链脂肪酸酯化。这些结果表明，米曲霉脂肪酶将是合成低热量油（如含有长链和短链脂肪酸混合物的三酰甘油）的有力生物催化剂。
