从牛膝中分离得到的五种具有抑制破骨细胞形成活性的新齐墩果酸糖苷。
Five new oleanolic acid glycosides from Achyranthes bidentata with inhibitory activity on osteoclast formation.
作者信息
Li Jian-Xin, Hareyama Toru, Tezuka Yasuhiro, Zhang Yuan, Miyahara Tatsuro, Kadota Shigetoshi
机构信息
Key Lab of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, P. R. China.
出版信息
Planta Med. 2005 Jul;71(7):673-9. doi: 10.1055/s-2005-871275.
Bioassay-directed fractionation of a butanol-soluble fraction of methanol extract of the root of Achyranthes bidentata resulted in the isolation of 5 new oleanolic acid glycosides 1-5, namely, 18-(beta-D-glucopyranosyloxy)-28-oxoolean-12-en-3beta-yl 3-O-(beta-D-glucopyranosyl)-beta-D-glucopyranosiduronic acid methyl ester (1), achyranthoside C dimethyl ester (2), achyranthoside C butyl dimethyl ester (3), achyranthoside E dimethyl ester (4), and achyranthoside E butyl methyl ester (5), together with 10 known compounds. Their structures were established on the basis of spectroscopic interpretation and chemical methods. All the oleanolic acid glycosides inhibited the formation of osteoclast-like multinucleated cells (OCLs) induced by 1alpha,25(OH)2D3 in a co-culture assay system.
对牛膝根甲醇提取物的正丁醇可溶部分进行生物活性导向的分离，得到了5个新的齐墩果酸糖苷1 - 5，即18 -（β - D - 吡喃葡萄糖氧基）- 28 - 氧代齐墩果 - 12 - 烯 - 3β - 基3 - O -（β - D - 吡喃葡萄糖基）-β - D - 吡喃葡萄糖醛酸甲酯（1）、牛膝苷C二甲酯（2）、牛膝苷C丁基二甲酯（3）、牛膝苷E二甲酯（4）和牛膝苷E丁基甲酯（5），以及10个已知化合物。它们的结构通过光谱解析和化学方法得以确定。在共培养测定系统中，所有的齐墩果酸糖苷均抑制了由1α,25(OH)2D3诱导的破骨细胞样多核细胞（OCLs）的形成。
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