Braga Sayonara Maria Lia Fook Meira, de Medeiros Francinalva Dantas, de Oliveira Eduardo Jesus, Macedo Rui Oliveira
UDEM/LTF, Universidade Federal da Paraíba, Caixa Postal 5044, CEP: 58051-970, João Pessoa, PB, Brazil.
Phytochem Anal. 2005 Jul-Aug;16(4):267-71. doi: 10.1002/pca.837.
A method for the quantification of aflatoxins B1, G1, B2 and G2 in the medicinal herb Maytenus ilicifolia was developed and validated. The method used immunoaffinity columns for sample clean-up and HPLC with fluorescence detection without any derivatisation step. The method showed good inter-day accuracy (bias values in the range 4.5-10.7%) and precision (5-16% RSD) when applied to the determination of levels of aflatoxins ranging from 7 to 20 ppb in the plant material. The detection limits for samples of the plant material spiked with aflatoxins were 3.5 ng/g for B1 and G1 and 0.1 ng/g for B2 and G2. The method was successfully applied to commercial samples of Maytenus ilicifolia for the screening of aflatoxin contaminants.
开发并验证了一种用于定量药用植物冬青叶美登木中黄曲霉毒素B1、G1、B2和G2的方法。该方法使用免疫亲和柱进行样品净化，并采用高效液相色谱法结合荧光检测，无需任何衍生步骤。当应用于测定植物材料中黄曲霉毒素含量在7至20 ppb范围内时，该方法显示出良好的日间准确性（偏差值在4.5 - 10.7%范围内）和精密度（相对标准偏差为5 - 16%）。添加了黄曲霉毒素的植物材料样品的检测限为：B1和G1为3.5 ng/g，B2和G2为0.1 ng/g。该方法已成功应用于冬青叶美登木的商业样品，用于黄曲霉毒素污染物的筛查。
