Laboratório de Micotoxinas, Fundação Ezequiel Dias , Belo Horizonte, MG , Brasil.
Braz J Microbiol. 2012 Oct;43(4):1428-36. doi: 10.1590/S1517-838220120004000026. Epub 2012 Jun 1.
The media claims for the consumption of natural resource-based food have gradually increased in both developing and developed countries. The interest in the safety of these products is partially due to the possible presence of toxigenic fungi acting as mycotoxin producers, such as aflatoxins produced during the secondary metabolism of Aspergillus flavus, A. parasiticus and A. nomius. Aflatoxins, mainly aflatoxin B1, are directly associated with liver cancer in human beings. This paper is aimed at evaluating the presence of aflatoxin B1 in a few vegetable drugs, dried plant extracts and industrialized products traded in 2010 in the city of Belo Horizonte, State of Minas Gerais, Brazil. The method used for the quantification of aflatoxin B1 was based on extraction through acetone:water (85:15), immunoaffinity column purification followed by separation and detection in high efficiency liquid chromatography. Under the conditions of analysis, the Limits of Detection and Quantification were 0.6 µg kg(-1) and 1.0 µg kg(-1) respectively. The complete sets of analyses were carried out in duplicate. Aflatoxin B1 was noticed in a single sample (< 1.0 µg kg(-1)). The results revealed low aflatoxin B1 contamination in the products under analysis. However, it is required to establish a broad monitoring program in order to obtain additional data and check up on the actual extension of contamination.
在发展中国家和发达国家，媒体对天然资源类食品的消费诉求逐渐增加。人们对这些产品安全性的关注部分是由于可能存在产毒真菌，如黄曲霉、寄生曲霉和构巢曲霉的次级代谢产物黄曲霉毒素。黄曲霉毒素主要是黄曲霉毒素 B1，与人类肝癌直接相关。本文旨在评估巴西米纳斯吉拉斯州贝洛奥里藏特市 2010 年贸易的几种蔬菜药材、干植物提取物和工业化产品中是否存在黄曲霉毒素 B1。用于定量检测黄曲霉毒素 B1 的方法基于丙酮：水（85:15）提取，免疫亲和柱净化，然后在高效液相色谱中分离和检测。在分析条件下，检测限和定量限分别为 0.6 µg kg(-1) 和 1.0 µg kg(-1)。所有分析均重复进行。仅在一个样本（< 1.0 µg kg(-1)）中发现了黄曲霉毒素 B1。结果表明，所分析的产品中黄曲霉毒素 B1 污染程度较低。但是，有必要建立一个广泛的监测计划，以获得更多的数据，并检查实际的污染范围。
