de Paiva Selma Ribeiro, Figueiredo Maria Raquel, Kaplan Maria Auxiliadora Coelho
Programa de Pós-Graduação em Biotecnologia Vegetal, Universidade Federal do Rio de Janeiro, 21941-590 Cidade Universitária, Rio de Janeiro, Brazil.
Phytochem Anal. 2005 Jul-Aug;16(4):278-81. doi: 10.1002/pca.841.
Centrifugal liquid-liquid partition chromatography presents significant advantages for the separation and purification of plant metabolites owing to the short operational time of the process and the elimination of possible irreversible adsorption of compounds. The crude chloroform extract from roots of Plumbago auriculata was analysed by countercurrent chromatography using hexane:ethyl acetate:methanol:water (40:10:10:2, v/v) as solvent system. The isolation of the naphthoquinones plumbagin and epi-isoshinanolone, the steroids sitosterol and 3-O-glucosylsitosterol, plumbagic and palmitic acids was easily achieved. Naphthoquinones are typical components of Plumbago species and they show interesting biological activities.
离心液-液分配色谱法在植物代谢物的分离和纯化方面具有显著优势，这归因于该过程操作时间短以及消除了化合物可能的不可逆吸附。以正己烷：乙酸乙酯：甲醇：水（40:10:10:2，v/v）为溶剂体系，采用逆流色谱法对耳草的根粗氯仿提取物进行了分析。萘醌类化合物白花丹醌和表异紫罗醇酮、甾体化合物甾醇和3-O-葡萄糖基甾醇、白花丹酸和棕榈酸很容易被分离出来。萘醌类是耳草属植物的典型成分，并且具有有趣的生物活性。
