Wang Lun, Zhang Li, She Shike, Gao Feng
College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2005 Sep;61(11-12):2737-40. doi: 10.1016/j.saa.2004.09.028. Epub 2004 Dec 8.
We established a simple and selective fluorimetric method for the determination of ascorbic acid (AA) with the novel mono-[6-N(4-carboxy-phenyl)]-beta-cyclodextrin (ACD). The method is based on the fluorescence intensity of ACD decreases as the ACD-AA supramolecular complexes form. The fluorescence intensity is measured at excitation and emission wavelengths of 270 and 352 nm, respectively. Under optimum condition, a linear relationship is obtained between the fluorescence intensity and the concentration of AA in the range of 0.05-8.0 microgml(-1). The detection limit is 0.012 microgml(-1). The method has been applied to the direct analysis of AA in real samples with satisfactory results.
我们建立了一种简单且具有选择性的荧光分析法，用于使用新型单-[6-N(4-羧基-苯基)]-β-环糊精（ACD）测定抗坏血酸（AA）。该方法基于随着ACD-AA超分子复合物形成，ACD的荧光强度降低。分别在激发波长270nm和发射波长352nm处测量荧光强度。在最佳条件下，荧光强度与AA浓度在0.05 - 8.0μg/ml范围内呈线性关系。检测限为0.012μg/ml。该方法已应用于实际样品中AA的直接分析，结果令人满意。
