Department of Chemistry, Yangzhou University, Yangzhou, Jiangsu 225002, People's Republic of China.
Spectrochim Acta A Mol Biomol Spectrosc. 2010 Oct 15;77(3):566-71. doi: 10.1016/j.saa.2010.04.028. Epub 2010 May 6.
A novel beta-cyclodextrin (beta-CD) sensitized fluorescence quenching method for the determination of l-tyrosine (l-Tyr) with Mo(VI)-phenyl-fluorone (PF) as a fluorescence probe has been developed. The fluorescence intensity of Mo(VI)-PF-beta-CD was diminished as the l-tyrosine was added, the fluorescence quenching value DeltaF=F(beta-CD-Mo-PF)-F(beta-CD-Mo-PF-l-Tyr) was enhanced in beta-CD and there was a linear relationship between the DeltaF and the concentration of l-Tyr. Under the optimal conditions, the linear range of calibration curve for the determination of l-tyrosine was 0.3-20.0microgmL(-1); the detection limit was 0.094microgmL(-1). NaOH (10%, w/v) is the best reagent of hydrolysis in sample preparation. The sensitized mechanism of beta-cyclodextrin was discussed. The method has been applied to the determination of l-tyrosine in spirulina and food samples with satisfactory results.
一种新型的β-环糊精（β-CD）敏化荧光猝灭法已被开发用于测定 L-酪氨酸（L-Tyr），其中 Mo(VI)-苯基荧光酮（PF）作为荧光探针。当加入 L-酪氨酸时，Mo(VI)-PF-β-CD 的荧光强度减弱，β-CD 中的荧光猝灭值ΔF=F（β-CD-Mo-PF）-F（β-CD-Mo-PF-L-Tyr）增强，并且ΔF 与 L-Tyr 的浓度之间存在线性关系。在最佳条件下，测定 L-酪氨酸的校准曲线的线性范围为 0.3-20.0μgmL(-1)；检测限为 0.094μgmL(-1)。NaOH（10%，w/v）是样品制备中水解的最佳试剂。讨论了β-环糊精的敏化机制。该方法已应用于螺旋藻和食品样品中 L-酪氨酸的测定，结果令人满意。
