Lum Z P, Krestow M, Tai I T, Vacek I, Sun A M
Department of Physiology, University of Toronto, Ontario, Canada.
Transplantation. 1992 Jun;53(6):1180-3. doi: 10.1097/00007890-199206000-00002.
Healthy rat islets were encapsulated in alginate-polylysine-alginate capsules measuring 0.25-0.35 mm in diameter using a modified encapsulation technique. The encapsulated islets were transplanted intraperitoneally in nonimmunosuppressed streptozotocin-induced diabetic BALB/c mice. The diabetic condition of the experimental animals was reversed within two days following the transplantation and the animals remained normoglycemic for up to 308 days, with a mean xenograft survival of 219.8 +/- 46.2 days. Four and six months posttransplant the capsules were removed from two recipients. This resulted in regression to a hyperglycemic state. After a second transplant of encapsulated islets, the animals returned to normoglycemia. In control mice that received free unencapsulated islets, the xenografts remained functional for no more than 12 days. Our study clearly demonstrates that the encapsulation of islets in the new smaller capsules can effectively prolong xenograft survival without immunosuppression.
使用改良的包封技术，将健康大鼠胰岛封装在直径为0.25 - 0.35毫米的海藻酸盐-聚赖氨酸-海藻酸盐胶囊中。将封装好的胰岛腹腔内移植到未进行免疫抑制的链脲佐菌素诱导的糖尿病BALB/c小鼠体内。移植后两天内，实验动物的糖尿病状态得到逆转，动物血糖正常长达308天，异种移植物平均存活时间为219.8±46.2天。移植后4个月和6个月，从两只受体动物体内取出胶囊。这导致动物恢复到高血糖状态。再次移植封装的胰岛后，动物又恢复到血糖正常状态。在接受游离未封装胰岛的对照小鼠中，异种移植物功能维持不超过12天。我们的研究清楚地表明，在新型较小胶囊中封装胰岛可在不进行免疫抑制的情况下有效延长异种移植物的存活时间。
