大肠杆菌K-12中四环素抗性的诱导与逆转以及质子梯度依赖性多药外排泵基因的表达
Inducement and reversal of tetracycline resistance in Escherichia coli K-12 and expression of proton gradient-dependent multidrug efflux pump genes.
作者信息
Viveiros Miguel, Jesus Ana, Brito Mafalda, Leandro Clara, Martins Marta, Ordway Diane, Molnar Ana Maria, Molnar Joseph, Amaral Leonard
机构信息
Unit of Mycobacteriology, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Rua Junqueira 96, 1349-008 Lisbon, Portugal.
出版信息
Antimicrob Agents Chemother. 2005 Aug;49(8):3578-82. doi: 10.1128/AAC.49.8.3578-3582.2005.
Abstract
Expression of eight transporter genes of Escherichia coli K-12 and its DeltaacrAB mutant prior to and after induction of both strains to tetracycline resistance and after reversal of induced resistance were analyzed by quantitative reverse transcriptase PCR. All transporter genes were overexpressed after induced resistance with acrF being 80-fold more expressed in the DeltaacrAB tetracycline-induced strain.
摘要
通过定量逆转录PCR分析了大肠杆菌K-12及其ΔacrAB突变体在诱导四环素抗性之前、诱导后以及诱导抗性逆转后8种转运蛋白基因的表达情况。在诱导抗性后，所有转运蛋白基因均过表达，其中acrF在ΔacrAB四环素诱导菌株中的表达量高出80倍。
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