Biernacki Michał, Brzóska Małgorzata Michalina, Markowska Agnieszka, Gałażyn-Sidorczuk Małgorzata, Cylwik Bogdan, Gęgotek Agnieszka, Skrzydlewska Elżbieta
Department of Analytical Chemistry, Medical University of Bialystok, A. Mickiewicza 2D, 15-222 Bialystok, Poland.
Department of Toxicology, Medical University of Bialystok, A. Mickiewicza 2C, 15-089 Bialystok, Poland.
Antioxidants (Basel). 2021 May 21;10(6):821. doi: 10.3390/antiox10060821.
UVA/UVB radiation disturbs the redox balance of skin cells, and metabolic consequences can be transferred into the blood and internal tissues, especially after chronic skin exposure to UV radiation. Therefore, the aim of this study was to evaluate the effect of cannabidiol (CBD), an antioxidant and anti-inflammatory phytocannabinoid, on oxidative stress and its consequences in the blood of nude rats whose skin was exposed to UVA/UVB radiation for 4 weeks. It was shown that CBD penetrated the blood and in UVB-irradiated rats was preferentially located in the membranes of polymorphonuclear leukocytes, which promoted reduction of ROS generation and up-regulation of antioxidant ability by increasing the activity of glutathione reductase and thioredoxin reductase, while the level of reduced glutathione decreased by UV radiation. Consequently, reduction in UV-induced lipid peroxidation, assessed as 4-hydroxynonenal (4-HNE) and 8-isoprostane (8-isoPGF2α) as well as protein modifications, estimated as 4-HNE-protein adducts and protein carbonyl groups, was observed. CBD, by countering the UV-induced down-regulation of 2-arachidonylglycerol, promoted its antioxidant/anti-inflammatory effects by reducing CB1 and increasing PPARγ receptor activation and consequently ROS and TNF-α down-regulation. The results suggest that CBD applied topically to the skin minimizes redox changes not only at the skin level, but also at the systemic level.
紫外线A/紫外线B辐射会扰乱皮肤细胞的氧化还原平衡，其代谢后果会传递到血液和内部组织中，尤其是在皮肤长期暴露于紫外线辐射之后。因此，本研究的目的是评估抗氧化和抗炎植物大麻素大麻二酚（CBD）对皮肤暴露于紫外线A/紫外线B辐射4周的裸鼠血液中氧化应激及其后果的影响。结果表明，CBD可进入血液，在紫外线B照射的大鼠中，它优先定位在多形核白细胞的膜中，这通过增加谷胱甘肽还原酶和硫氧还蛋白还原酶的活性促进了活性氧生成的减少和抗氧化能力的上调，而紫外线辐射使还原型谷胱甘肽水平降低。因此，观察到紫外线诱导的脂质过氧化（以4-羟基壬烯醛（4-HNE）和8-异前列腺素（8-isoPGF2α）评估）以及蛋白质修饰（以4-HNE-蛋白质加合物和蛋白质羰基基团评估）减少。CBD通过对抗紫外线诱导的2-花生四烯酸甘油下调，通过减少CB1并增加过氧化物酶体增殖物激活受体γ（PPARγ）受体激活，从而减少活性氧和肿瘤坏死因子-α（TNF-α）的下调，促进了其抗氧化/抗炎作用。结果表明，局部应用于皮肤的CBD不仅可使皮肤水平的氧化还原变化最小化，还可使全身水平的氧化还原变化最小化。
